Effects of curcumin on antioxidative activities and cytokine production in Jian carp (Cyprinus carpio var. Jian) with CCl4-induced liver damage
Effects of curcumin on antioxidative activities and cytokine production in Jian carp (Cyprinus carpio var. Jian) with CCl4-induced liver damage
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DOI：
10.1016/j.fsi.2014.12.025
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发表时间：
2015-03-01
影响因子：
4.7
通讯作者：
Yin, Guojun
中科院分区：
文献类型：
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作者：
Cao, Liping;Ding, Weidong;Yin, Guojun
研究了姜黄素对鲤鱼肝损伤的保护作用。建（建鲤）。分别用0.1%、0.5%和1.0%姜黄素饲喂鲤鱼60 d，然后腹腔注射30%四氯化碳溶液。采集肝脏和血液样本，以测量肝脏指数、血清和肝脏相关酶、肝脏组织学、核因子-κ B（NF-κ B）/c-Rel、白细胞介素-1 β（IL-1 β）、肿瘤坏死因子-α（TNF-α）和IL-12 mRNA表达，以及肝脏中NF-κ B/c-Rel蛋白水平，并进行彗星试验。我们发现，0.5%和1.0%姜黄素显着降低四氯化碳诱导的肝脏指数增加。彗星试验表明，尾矩，橄榄尾矩，尾长，尾DNA%在鱼用0.5或1.0%姜黄素预处理提高。用0.5%和1.0%姜黄素处理后，CCl 4诱导的组织学变化，包括广泛的肝细胞变性、细胞边界模糊、核浓缩和核溶解明显减少。此外，0.5%和1.0%姜黄素可显著抑制四氯化碳诱导的血清谷草转氨酶升高，促进肝脏超氧化物歧化酶的恢复; 1.0%姜黄素可显著降低血清谷草转氨酶和乳酸脱氢酶以及肝脏丙二醛，但显著提高肝脏总抗氧化能力和谷胱甘肽水平。CCl 4诱导的NF-κ B/c-Rel、IL-1 β和TNF-α mRNA以及NF-κ B/c-Rel蛋白水平的上调被0.5%和1.0%姜黄素抑制，并且IL-12 mRNA被所有三种剂量的姜黄素降低。姜黄素对肝脏指数、酶、组织学变化和细胞因子的影响呈剂量依赖性。我们的研究结果表明，姜黄素通过上调抗氧化活性和抑制NF-κ B B、IL-1 β、TNF-α和IL-12的表达来减轻CCl 4诱导的建鲤肝损伤。(C)2014爱思唯尔有限公司版权所有。
We investigated the protective effects of curcumin on liver-damaged Cyprinus carpio var. Jian (Jian carp). The carp were fed 0.1%, 0.5%, or 1.0% curcumin for 60 days, then injected intraperitoneally with 30% carbon tetrachloride solution. Liver and blood samples were collected to measure the liver index, serum- and liver-associated enzymes, liver histology, nuclear factor-kappa B (NF-kappa B)/c-Rel, interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and IL-12 mRNA expression, and the level of NF-kappa B/c-Rel protein in the liver, and for a comet assay. We found that 0.5% and 1.0% curcumin significantly reduced the CCl4-induced increase in the liver index. The comet assay showed that the tail moment, olive tail moment, tail length, and tail DNA% improved in fish pretreated with 0.5 or 1.0% curcumin. CCl4-induced histological changes, including extensive hepatocyte degeneration, indistinct cell borders, nuclear condensation, and karyolysis were clearly reduced after treatment with 0.5% and 1.0% curcumin. Moreover, 0.5% and 1.0% curcumin significantly inhibited the CCl4-induced increase in serum glutamic oxaloacetic transaminase and promoted the restoration of superoxide dismutase in the liver; 1.0% curcumin significantly reduced serum glutamic pyruvic transaminase and lactate dehydrogenase and hepatic malondialdehyde, but significantly increased the total antioxidant capacity and glutathione levels in the liver. The CCl4-induced upregulation of NF-kappa B/c-Rel, IL-1 beta, and TNF-alpha mRNAs and NF-kappa B/c-Rel protein levels was inhibited by 0.5% and 1.0% curcumin, and IL-12 mRNA was reduced by all three doses of curcumin. The effects of curcumin on the liver index, enzymes, histological changes, and cytokines were dose-dependent. Our results indicate that curcumin reduces CCl4-induced liver damage in Jian carp by upregulating antioxidative activities and inhibiting NF-kappa B, IL-1 beta, TNF-alpha, and IL-12 expression. (C) 2014 Elsevier Ltd. All rights reserved.