IDENTIFICATION AND CLONING OF A NOVEL IL-15 BINDING-PROTEIN THAT IS STRUCTURALLY RELATED TO THE ALPHA-CHAIN OF THE IL-2 RECEPTOR
IDENTIFICATION AND CLONING OF A NOVEL IL-15 BINDING-PROTEIN THAT IS STRUCTURALLY RELATED TO THE ALPHA-CHAIN OF THE IL-2 RECEPTOR
复制标题
DOI：
10.1002/j.1460-2075.1995.tb00035.x
复制
发表时间：
1995-08-01
期刊：
影响因子：
11.4
通讯作者：
ANDERSON, DM
中科院分区：
文献类型：
--
作者：
GIRI, JG;KUMAKI, S;ANDERSON, DM
白细胞介素-15（IL-15）是四螺旋束家族的新细胞因子，其与IL-2共享许多生物学活性，可能是由于其与IL-2受体β和γ（IL-2 R β和γ（c））链的相互作用。我们在这里报告的表征和分子克隆的一个独特的小鼠IL-15 R α链。单独的IL-15 R α显示出与异源三聚体IL-2 R对IL-2的结合亲和力相当的对IL-15的结合亲和力。通过在鼠骨髓细胞系中的重建实验产生能够介导IL-15应答的生物学功能异聚体IL-15受体复合物。IL-15 R α在结构上与IL-2 R α相似;它们一起定义了一个新的细胞因子受体家族。IL-15和IL-15 R α mRNA的分布表明IL-15可能具有不同于IL-2的生物活性。
Interleukin-15 (IL-15) is a novel cytokine of the four-helix bundle family which shares many biological activities with IL-2, probably due to its interaction with the IL-2 receptor beta and gamma (IL-2R beta and gamma(c)) chains. We report here the characterization and molecular cloning of a distinct murine IL-15R alpha chain. IL-15R alpha alone displays an affinity of binding for IL-15 equivalent to that of the heterotrimeric IL-2R for IL-2. A biologically functional heteromeric IL-15 receptor complex capable of mediating IL-15 responses was generated through reconstruction experiments in a murine myeloid cell line. IL-15R alpha is structurally similar to IL-2R alpha; together they define a new cytokine receptor family. The distribution of IL-15 and IL-15R alpha mRNA suggests that IL-15 may have biological activities distinct from IL-2.