Construction of a fosmid library of cucumber (Cucumis sativus) and comparative analyses of the eIF4E and eIF(iso)4E regions from cucumber and melon (Cucumis melo)
Construction of a fosmid library of cucumber (Cucumis sativus) and comparative analyses of the eIF4E and eIF(iso)4E regions from cucumber and melon (Cucumis melo)
复制标题
DOI：
10.1007/s00438-008-0326-5
复制
发表时间：
2008-02
影响因子：
3.1
通讯作者：
Jenelle D. F. Meyer;Wim Deleu;J. Garcia-Mas;M. Havey
中科院分区：
文献类型：
--
作者：
Jenelle D. F. Meyer;Wim Deleu;J. Garcia-Mas;M. Havey
合成了黄瓜的 fosmid 文库，作为研究人员的不受限制的资源，并用于比较序列分析，以评估黄瓜和甜瓜基因组之间的同线性，这两种基因组都是黄瓜属的成员，也是葫芦科中两种最重要的经济植物。随机fosmids的末端测序产生了超过680kb的黄瓜基因组序列，其中25%与核糖体DNA相似，25%与卫星序列相似，20%与其他植物的编码区相似，4%与转座元件相似，13%与线粒体和叶绿体序列相似，13%与数据库没有匹配。核糖体和卫星 DNA 的相对较高频率与之前对黄瓜 DNA 的分析一致。选择携带真核起始因子 (eIF) 4E 和 iso(4E) 的黄瓜 fosmid 并对其进行测序，这些基因与许多植物中对马铃薯病毒属隐性遗传抗性相关。 eIF4E 和 eIF(iso)4E 附近的插入缺失与 thezym 无关，这是一个隐性基因座，调节对西葫芦黄花叶病毒的抗性，确定这些候选基因不是zym。将黄瓜序列与携带 eIF4E 和 eIF(iso)4E 的甜瓜 BAC 进行比较，揭示了黄瓜和甜瓜在这两个独立基因组区域之间广泛的序列保守性和同线性。这种高度的微同线性将有助于克隆两个物种的直系同源基因，并允许为一个黄瓜物种开发的基因组资源用于其他物种的分析。
A fosmid library of cucumber was synthesized as an unrestricted resource for researchers and used for comparative sequence analyses to assess synteny between the cucumber and melon genomes, both members of the genusCucumisand the two most economically important plants in the family Cucurbitaceae. End sequencing of random fosmids produced over 680 kilobases of cucumber genomic sequence, of which 25% was similar to ribosomal DNAs, 25% to satellite sequences, 20% to coding regions in other plants, 4% to transposable elements, 13% to mitochondrial and chloroplast sequences, and 13% showed no hits to the databases. The relatively high frequencies of ribosomal and satellite DNAs are consistent with previous analyses of cucumber DNA. Cucumber fosmids were selected and sequenced that carried eukaryotic initiation factors (eIF) 4E and iso(4E), genes associated with recessively inherited resistances to potyviruses in a number of plants. Indels near eIF4E and eIF(iso)4E mapped independently of thezym, a recessive locus conditioning resistance toZucchini yellow mosaic virus, establishing that these candidate genes are notzym. Cucumber sequences were compared with melon BACs carrying eIF4E and eIF(iso)4E and revealed extensive sequence conservation and synteny between cucumber and melon across these two independent genomic regions. This high degree of microsynteny will aid in the cloning of orthologous genes from both species, as well as allow for genomic resources developed for oneCucumisspecies to be used for analyses in other species.