AN ANTISENSE MESSENGER-RNA DIRECTS THE COVALENT MODIFICATION OF THE TRANSCRIPT ENCODING FIBROBLAST GROWTH-FACTOR IN XENOPUS OOCYTES
AN ANTISENSE MESSENGER-RNA DIRECTS THE COVALENT MODIFICATION OF THE TRANSCRIPT ENCODING FIBROBLAST GROWTH-FACTOR IN XENOPUS OOCYTES
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DOI：
10.1016/0092-8674(89)90015-9
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发表时间：
1989-11-17
期刊：
影响因子：
64.5
通讯作者：
KIRSCHNER, MW
中科院分区：
文献类型：
--
作者：
KIMELMAN, D;KIRSCHNER, MW
非洲爪哇卵母细胞含有三个母体碱性成纤维细胞生长因子(BFGF)转录本，其中最大的转录本编码完整的碱性成纤维细胞生长因子多肽。我们发现最小的转录本与最大的转录本的转录方向相反，并与bFGF的部分编码序列重叠。该反义转录本编码一个高度保守的25kd多肽。此外，反义转录本在卵母细胞成熟过程中会导致编码bFGFmRNA的修饰，在正义和反义转录本重叠的区域将一半的腺嘌呤残基转化为肌苷。由于这种活性只作用于双链RNA，这两个mRNAs必须在卵母细胞中杂交。我们讨论了反义转录本在调节bFGFm RNA稳定性中的可能作用。
The Xenopus oocyte contains three maternal basic fibroblast growth factor (bFGF) transcripts, the largest of which encodes the complete bFGF polypeptide. We show that the smallest transcript is transcribed in the opposite direction of the largest transcript and overlaps part of the coding sequence of bFGF. This antisense transcript encodes a highly conserved 25 kd polypeptide. In addition, the antisense transcript causes modification of the mRNA encoding bFGF during maturation of the oocyte, converting half of the adenine residues to inosine in the region of overlap between the sense and antisense transcripts. As this activity acts only on double-stranded RNA, the two mRNAs must be hybridized in the oocyte. We discuss a possible role for the antisense transcript in regulating the stability of the bFGF mRNA.