Opa interacting protein 5 (OIP5) is a novel cancer-testis specific gene in gastric cancer
Opa interacting protein 5 (OIP5) is a novel cancer-testis specific gene in gastric cancer
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DOI：
10.1245/s10434-006-9121-x
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发表时间：
2007-02-01
影响因子：
3.7
通讯作者：
Mori, Masaki
中科院分区：
文献类型：
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作者：
Nakamura, Yoshito;Tanaka, Fumiaki;Mori, Masaki
背景：新的肿瘤特异性抗原的鉴定对免疫治疗的发展具有重要意义。我们的目的是寻找胃癌中的肿瘤特异性基因。方法：利用基因芯片技术，检测胃癌细胞中高表达的基因。通过对58例临床病例的肿瘤/正常配对样本进行实时定量RT-PCR分析，证实了包括OIP5在内的选定基因的表达水平。选择的基因在正常组织中的表达水平也用人类总RNA主板进行了测定。我们还比较了OIP5与其他已知的肿瘤-睾丸特异基因的表达情况。结果：确定了22个基因在胃癌细胞中上调。其中有3个基因(CDC6、Exo1和OIP5)在肿瘤组织中与正常组织相比表达上调。人类总RNA主板表明，OIP5，而不是Exo1或CDC6，在睾丸中显示出高度的特异性。因此，OIP5可能被认为是一个肿瘤-睾丸特异基因。在58例胃癌临床标本中，27例(47%)有OIP5基因表达。其中13例未检测到已知的肿瘤特异性基因MAGE-1、MAGE-3或NY-ESO-1。结论：结合LMD和基因芯片技术，OIP5为睾丸肿瘤特异性基因。对一组临床病例的进一步表达分析表明，OIP5可能是胃癌患者的一个新的免疫治疗靶点。
Background: Identification of novel cancer-specific antigens is important for the advancement of immunotherapy. Our aim was to identify cancer-specific genes in gastric cancer.Methods: Using cDNA microarray analysis, we detected genes overexpressed specifically in gastric cancer cells. The expression levels of selected genes, including OIP5, was confirmed by real time RT-PCR analysis in tumor/normal paired bulk samples of 58 clinical cases. The expression levels of selected genes in normal tissues were also determined with a human total RNA master panel. We also compared the expression status of OIP5 with that of the other known cancer-testis specific genes.Results: Twenty-two genes were determined to be upregulated in gastric cancer cells. Among these, three genes (CDC6, Exo1, and OIP5) were selected and confirmed to be upregulated in the tumor tissue compared to normal tissue. A human total RNA master panel demonstrated that OIP5, but not Exo1 or CDC6, showed high specificity in testis. Thus OIP5 may be considered a cancer-testis specific gene. In 58 clinical cases of gastric cancer examined, we found OIP5 gene expression in 27 cases (47%). Thirteen of these 27 cases showed no expression of the known cancer specific genes such as MAGE-1, MAGE-3 or NY-ESO-1.Conclusions: Using a combination of LMD and microarray, we identified OIP5 as a cancer-testis specific gene. Further expression analysis in a set of clinical cases revealed that OIP5 may be a novel immunotherapy target for patients with gastric cancer.