Effects of Low Frequency Electromagnetic Fields on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells
Effects of Low Frequency Electromagnetic Fields on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells
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DOI：
10.1002/bem.20633
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发表时间：
2011-05-01
影响因子：
1.9
通讯作者：
Mueller, Peter E.
中科院分区：
文献类型：
--
作者：
Mayer-Wagner, Susanne;Passberger, Alice;Mueller, Peter E.
电磁场（EMF）已被证明对软骨组织有有益的作用。目前，分化的人间充质干细胞（hMSCs）被认为是软骨修复的一种替代方法。因此，本研究的目的是研究EMF在软骨分化过程中对hMSCs的影响。在添加人成纤维细胞生长因子2 （FGF-2）和人转化生长因子- β (tgf - β(3))的条件下，体外培养第5代和第6代的HMSCs。将培养物暴露在由螺线管产生的均匀正弦极低频磁场（5mt）中或保存在控制系统中。培养3周后，通过甲苯胺蓝和红花素o染色、免疫组织化学、软骨特异性蛋白的实时定量聚合酶链反应（PCR）和糖胺聚糖的DMMB染料结合试验来评估软骨形成。在EMF作用下，hMSCs在传代6时II型胶原的表达显著增加。EMF暴露后Aggrecan和SOX9的表达无明显变化。X型胶原蛋白在电磁刺激下表达降低。与未暴露于EMF的培养物相比，经EMF处理的第5代颗粒培养物提供了更高的糖胺聚糖(GAG)/DNA含量。EMF对培养物中II型胶原表达和GAG含量的影响可能会促进hMSCs的软骨分化。EMF可能是一种刺激和维持hMSCs软骨形成的方法，因此，在软骨组织工程的再生医学中提供了一个新的步骤。中国生物医学工程学报，2011,31(2):387 - 398。(c) 2010 Wiley-Liss, Inc。
Electromagnetic fields (EMF) have been shown to exert beneficial effects on cartilage tissue. Nowadays, differentiated human mesenchymal stem cells (hMSCs) are discussed as an alternative approach for cartilage repair. Therefore, the aim of this study was to examine the impact of EMF on hMSCs during chondrogenic differentiation. HMSCs at cell passages five and six were differentiated in pellet cultures in vitro under the addition of human fibroblast growth factor 2 (FGF-2) and human transforming growth factor-beta(3) (TGF-beta(3)). Cultures were exposed to homogeneous sinusoidal extremely low-frequency magnetic fields (5 mT) produced by a solenoid or were kept in a control system. After 3 weeks of culture, chondrogenesis was assessed by toluidine blue and safranin-O staining, immunohistochemistry, quantitative real-time polymerase chain reaction (PCR) for cartilage-specific proteins, and a DMMB dye-binding assay for glycosaminoglycans. Under EMF, hMSCs showed a significant increase in collagen type II expression at passage 6. Aggrecan and SOX9 expression did not change significantly after EMF exposure. Collagen type X expression decreased under electromagnetic stimulation. Pellet cultures at passage 5 that had been treated with EMF provided a higher glycosaminoglycan (GAG)/DNA content than cultures that had not been exposed to EMF. Chondrogenic differentiation of hMSCs may be improved by EMF regarding collagen type II expression and GAG content of cultures. EMF might be a way to stimulate and maintain chondrogenesis of hMSCs and, therefore, provide a new step in regenerative medicine regarding tissue engineering of cartilage. Bioelectromagnetics 32: 283-290, 2011. (c) 2010 Wiley-Liss, Inc.