Design and Construction of Higher-Order Structure and Function in Proteinosome-Based Protocells
Design and Construction of Higher-Order Structure and Function in Proteinosome-Based Protocells
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DOI：
10.1021/ja504213m
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发表时间：
2014-06-25
影响因子：
15
通讯作者：
Mann, Stephen
中科院分区：
文献类型：
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作者：
Huang, Xin;Patil, Avinash J.;Mann, Stephen
描述了由两亲性牛血清白蛋白/聚（N-异丙基丙烯酰胺）（BSA-NH 2/PNIPAAm）纳米缀合物的交联膜包围的蛋白质体微区室中的高阶结构和功能的设计和构建。研究了三种结构/功能关系：（i）用于控制膜分解和包封的遗传聚合物的调节释放的差异化学交联;（ii）内部微环境的酶介导的水凝胶结构化，以增加机械鲁棒性并产生分子拥挤的反应环境;和（iii）自我产生包封膜的水凝胶外壁，用于产生蛋白酶抗性形式的蛋白质-聚合物原细胞。我们的研究结果突出了将超分子和聚合物化学方面整合到新型生物启发微区室的设计和构建中的潜力，作为基于合成细胞结构的小规模材料系统的一步。
The design and construction of higher-order structure and function in proteinosome microcompartments enclosed by a cross-linked membrane of amphiphilic bovine serum albumin/poly(N-isopropylacrylamide) (BSA-NH2/PNIPAAm) nanoconjugates is described. Three structure/function relationships are investigated: (i) differential chemical cross-linking for the control of membrane disassembly and regulated release of encapsulated genetic polymers; (ii) enzyme-mediated hydrogel structuring of the internal microenvironment to increase mechanical robustness and generate a molecularly crowded reaction environment; and (iii) self-production of a membrane-enclosing outer hydrogel wall for generating protease-resistant forms of the protein-polymer protocells. Our results highlight the potential of integrating aspects of supramolecular and polymer chemistry into the design and construction of novel bioinspired microcompartments as a step toward small-scale materials systems based on synthetic cellularity.