Expression of human β-defensin 2 in human nasal mucosa
Expression of human β-defensin 2 in human nasal mucosa
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DOI：
10.1007/s00405-003-0682-z
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发表时间：
2004-05-01
影响因子：
2.6
通讯作者：
Fang, SY
中科院分区：
文献类型：
--
作者：
Chen, PH;Fang, SY
人类β -防御素(HBD)-2是一种抗菌肽，已被发现由许多上皮细胞产生。它被认为是先天宿主防御机制中的一个关键因素。由于目前对HBD-2在人鼻道中的表达知之甚少，我们通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学定位HBD-2肽，研究了HBD-2 mRNA基因在人鼻下鼻甲和鼻息肉中的表达情况。RT-PCR结果显示，鼻息肉组织中HBD-2 mRNA的表达明显高于下鼻甲组织。免疫组化发现，HBD-2肽主要定位于表面上皮细胞。因此，HBD-2在鼻黏膜中表达并在慢性炎症状态下上调是可行的。
Human beta-defensin (HBD)-2, an antimicrobial peptide, has been discovered to be produced by a number of epithelial cells. It is identified as a key element in the innate host defense mechanism. Because little is known about the expression of HBD-2 in the human sinonasal tract, we conducted this study to investigate the expression of the HBD-2 mRNA gene by the reverse transcription polymerase chain reaction (RT-PCR) and localization of HBD-2 peptide by immunohistochemistry in human nasal inferior turbinates and nasal polyps. RT-PCR showed significantly higher expression of HBD-2 mRNA in nasal polyps than in inferior turbinates. Using immunohistochemistry, HBD-2 peptide was predominately localized in surface epithelial cells. Thus, it is feasible that HBD-2 is expressed in nasal mucosa and is upregulated in a condition of chronic inflammation.