Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
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DOI：
10.1046/j.1462-2920.2002.00370.x
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发表时间：
2002-12-01
影响因子：
5.1
通讯作者：
Díaz, E
中科院分区：
文献类型：
--
作者：
Jiménez, JI;Miñambres, B;Díaz, E
恶臭假单胞菌KT 2440对多种天然芳香化合物的分解代谢潜力的分析和与该微生物的整个基因组的序列比较预测了至少存在四种主要途径来催化中心芳香中间体，即原儿茶酸β-酮己二酸途径的分支（PCA基因）和儿茶酚（CAT基因）、尿黑酸途径（HMG /FAH /MAI基因）和苯乙酸途径（PHA基因）。另外两个可能参与N-杂环芳香族化合物催化的基因簇（nic簇）和一个中心的间位裂解途径（pcm基因）也被确定。此外，对羟基苯甲酸酯（pob）、苯甲酸酯（ben）、奎尼酸酯（qui）、苯丙烯类化合物（fcs、ech、vdh、cal、货车、acd和acs）、苯丙氨酸和酪氨酸（phh、hpd）和n-苯基链烷酸（fad）的催化剂的外周途径的编码基因定位于恶臭假单胞菌KT 2440的染色体中。尽管重复的基因外回文（REP）元件通常与基因簇相关，但在恶臭假单胞菌KT 2440中未观察到将不同芳香族化合物引入共同中心途径（分解代谢岛）的分解代谢基因的超操纵子簇。恶臭假单胞菌KT 2440对芳香族化合物矿化的全局观点将促进该菌株的合理操作，以改善生物降解/生物转化过程，并揭示该细菌作为研究芳香族化合物催化剂的生物化学，遗传，进化和生态学方面的有用模型系统。
Analysis of the catabolic potential of Pseudomonas putida KT2440 against a wide range of natural aromatic compounds and sequence comparisons with the entire genome of this microorganism predicted the existence of at least four main pathways for the catabolism of central aromatic intermediates, that is, the protocatechuate (pca genes) and catechol (cat genes) branches of the beta-ketoadipate pathway, the homogentisate pathway (hmg /fah /mai genes) and the phenylacetate pathway (pha genes). Two additional gene clusters that might be involved in the catabolism of N-heterocyclic aromatic compounds (nic cluster) and in a central meta-cleavage pathway (pcm genes) were also identified. Furthermore, the genes encoding the peripheral pathways for the catabolism of p-hydroxybenzoate (pob), benzoate (ben), quinate (qui), phenylpropenoid compounds (fcs, ech, vdh, cal, van, acd and acs), phenylalanine and tyrosine (phh , hpd ) and n-phenylalkanoic acids (fad) were mapped in the chromosome of P. putida KT2440. Although a repetitive extragenic palindromic (REP) element is usually associated with the gene clusters, a supraoperonic clustering of catabolic genes that channel different aromatic compounds into a common central pathway (catabolic island) was not observed in P. putida KT2440. The global view on the mineralization of aromatic compounds by P. putida KT2440 will facilitate the rational manipulation of this strain for improving biodegradation/biotransformation processes, and reveals this bacterium as a useful model system for studying biochemical, genetic, evolutionary and ecological aspects of the catabolism of aromatic compounds.