THE LINEAR PCR REACTION - A SIMPLE AND ROBUST METHOD FOR SEQUENCING AMPLIFIED RIBOSOMAL-RNA GENES
THE LINEAR PCR REACTION - A SIMPLE AND ROBUST METHOD FOR SEQUENCING AMPLIFIED RIBOSOMAL-RNA GENES
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DOI：
10.1111/j.1472-765x.1991.tb00600.x
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发表时间：
1991-01-01
影响因子：
2.4
通讯作者：
EMBLEY, TM
中科院分区：
文献类型：
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作者：
EMBLEY, TM
采用线性聚合酶链反应对芽孢杆菌、热菌和军团菌扩增的RNA基因进行测序。所描述的技术简单，重复性好，对PCR反应中直接沉淀的聚乙二醇双标准产物效果良好。
The linear polymerase chain reaction was used to sequence amplified RNA genes from strains of Bacillus, Thermus and Legionella. The technique described is simple and reproducible and it works well with double standard product which has been PEG precipitated directly from PCR reactions.