Analysis of endogenous cortisol concentrations in the hair of rhesus macaques
Analysis of endogenous cortisol concentrations in the hair of rhesus macaques
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DOI：
10.1016/j.ygcen.2006.01.005
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发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Meyer, Jerrold S.
中科院分区：
文献类型：
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作者：
Davenport, Matthew D.;Tiefenbacher, Stefan;Meyer, Jerrold S.
通常通过测量血浆、唾液、尿液或粪便中的糖皮质激素或代谢物浓度来评估下丘脑-垂体-肾上腺皮质 (HPA) 系统活性的短期变化。然而，目前没有方法来确定该系统的长期（即几周或几个月）活动。在此，我们描述了测量恒河猴毛发中皮质醇浓度的简单程序的开发和验证。该程序包括对发丝进行两次短暂的异丙醇洗涤，以去除表面污染物，随后将洗涤后的头发打粉并干燥，进行 24 小时甲醇提取，然后蒸发溶剂并在测定缓冲液中重新配制提取物，最后通过灵敏且特异的酶免疫分析对提取的皮质醇进行分析。我们的结果证实了皮质醇程序的特异性，表明头发的近端和远端部分的皮质醇浓度没有差异，并证明显着且长期的压力经历会导致头发皮质醇显着增加。这一新程序对于评估非人类灵长类动物（以及在适当的验证下，其他物种）在相对较长时间内的基线 HPA 活性，以及监测可能与各种实验操作相关的慢性应激，应该是有价值的。 (c) 2006 Elsevier Inc. 保留所有权利。
Short-term changes in activity of the hypothalamic-pituitary-adrenocortical (HPA) system are routinely assessed by measuring glucocorticoid or metabolite concentrations in plasma, saliva, urine, or feces. However, there are no current methods for determining long-term (i.e., weeks or months) activity of this system. Herein, we describe the development and validation of a simple procedure for measuring cortisol concentrations in the hair of rhesus macaques. This procedure involves two brief isopropanol washes of the hair strands to remove surface contaminants, subsequent powdering of the washed and dried hair, a 24-h methanol extraction followed by evaporation of the solvent and reconstitution of the extract in assay buffer, and finally analysis of the extracted cortisol by a sensitive and specific enzyme immunoassay. Our results confirm the specificity of the procedure for cortisol, show that proximal and distal segments of hair do not differ in their cortisol concentration, and demonstrate that a significant and prolonged stressful experience produces a significant increase in hair cortisol. This new procedure should be valuable for assessing baseline HPA activity in nonhuman primates (and, with appropriate validation, in other species as well) over relatively long periods of time, and also for monitoring chronic stress that might be associated with various experimental manipulations. (c) 2006 Elsevier Inc. All rights reserved.