Thermo-Responsive Glycopolymer Chains Grafted onto Honeycomb Structured Porous Films via RAFT Polymerization as a Thermo-Dependent Switcher for Lectin Concanavalin A Conjugation
Thermo-Responsive Glycopolymer Chains Grafted onto Honeycomb Structured Porous Films via RAFT Polymerization as a Thermo-Dependent Switcher for Lectin Concanavalin A Conjugation
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DOI：
10.1002/pola.24129
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发表时间：
2010-08-01
影响因子：
--
通讯作者：
Stenzel, Martina H.
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文献类型：
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作者：
Min, Eun Hee;Ting, S. R. Simon;Stenzel, Martina H.
通过交联聚苯乙烯-共马来酸酐制备蜂窝结构多孔膜，制备了具有规则多孔阵列的稳定和表面改性薄膜。薄膜的开孔或闭孔的形成是由多离子络合物的加入控制的。随后将膜与1,8-二氨基辛烷交联得到的膜在溶剂中保持其结构。此外，交联后过量的氨基功能允许RAFT剂3-苄基磺酰硫羰基磺酰丙酸的附着，用于n -异丙基丙烯酰胺（NIPAAm）和n -丙烯酰氨基葡萄糖（AGA）的受控表面聚合。利用接触角测量和共聚焦荧光显微镜证实了热响应型糖共聚物在蜂窝结构多孔膜上的附着。通过氨解对表面锚定聚合物的裂解表明，表面接枝链的分子量明显大于溶液中生成的链的分子量。接枝PNI-PAAm-ran-PAGA聚合物链的蜂窝结构多孔膜对ConA有选择性识别。在表面的较低临界溶液温度（LCST）以下，缀合作用被关闭，而在LCST以上，表面接枝的葡萄糖部分与ConA结合强烈。(C) 2010 Wiley期刊公司高分子材料学报，2010,31 (4):444 - 444
Stable and surface-modified films with regular porous arrays were created by crosslinking honeycomb structured porous films prepared via breath figures from poly(styrene-co-maleic anhydride). The formation of open or closed pores of the films was controlled by the addition of a polyion complex. Subsequent crosslinking of the films with 1,8-diaminooctane led to films, which maintain their structure in solvents. In addition, excess amino functionality after crosslinking allowed the attachment of RAFT agent, 3-benzylsulfanyl thiocarbonyl sulfanylpropionic acid, for the controlled surface polymerization of N-isopropyl acrylamide (NIPAAm) and N-acryloyl glucosamine (AGA). The attachment of thermo-responsive glycopolymers onto the honeycomb structured porous films was confirmed using contact angle measurements and confocal fluorescence microscopy. Cleavage of surface anchored polymers via aminolysis revealed that the molecular weights of the surface grafted chains are significantly larger than the molecular weight of the chains generated in solution. The honeycomb structured porous films with their grafted PNI-PAAm-ran-PAGA polymer chains showed selective recognition of Concanavalin A (ConA). Below the lower critical solution temperature (LCST) of the surface, the conjugation is switched off, while above the LCST the surface grafted glucose moieties bind strongly to ConA. (C) 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 3440-3455, 2010