De Novo Biosynthesis of Chlorogenic Acid Using an Artificial Microbial Community
De Novo Biosynthesis of Chlorogenic Acid Using an Artificial Microbial Community
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DOI：
10.1021/acs.jafc.0c07588
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发表时间：
2021-02-25
影响因子：
6.1
通讯作者：
Tang, Shuang-Yan
中科院分区：
文献类型：
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作者：
Li, Shizhong;Liang, Chaoning;Tang, Shuang-Yan
设计用于天然产物生产的人工微生物群落是一种有前途的策略。由于单和双培养系统只提供不可检测或最小的绿原酸（CGA）的生物合成，在这里，三个重组大肠杆菌菌株，作为咖啡酸（CA），奎尼酸（QA），和CGA的生物合成模块的混合培养物，被设计和用于从头CGA生物合成。将3-脱氢莽草酸（DHS）/莽草酸（SA）的内流转运蛋白ShiA引入到QA模块中。混合培养和CGA生产中的QA模块比例被发现依赖于ShiA表达，提供了一种控制微生物群落组成的替代方法。混合培养策略避免了两种CGA前体CA和QA的生物合成中的代谢通量竞争，并通过平衡模块比例来提高产量。这种混合培养方法的性能是上级的从头CGA生产的先前报道的方法。
Engineering an artificial microbial community for natural product production is a promising strategy. As mono- and dual-culture systems only gave non-detectable or minimal chlorogenic acid (CGA) biosynthesis, here, a polyculture of three recombinant Escherichia coli strains, acting as biosynthetic modules of caffeic acid (CA), quinic acid (QA), and CGA, was designed and used for de novo CGA biosynthesis. An influx transporter of 3-dehydroshikimic acid (DHS)/shikimic acid (SA), ShiA, was introduced into the QA module-a DHS auxotroph. The QA module proportion in the polyculture and CGA production were found to be dependent on ShiA expression, providing an alternative approach for controlling microbial community composition. The polyculture strategy avoids metabolic flux competition in the biosynthesis of two CGA precursors, CA and QA, and allows production improvement by balancing module proportions. The performance of this polyculture approach was superior to that of previously reported approaches of de novo CGA production.