Voltage- and γ-aminobutyric acid-activated membrane currents in the human medulloblastoma cell line MHH-MED-3
Voltage- and γ-aminobutyric acid-activated membrane currents in the human medulloblastoma cell line MHH-MED-3
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DOI：
10.1016/s0304-3940(00)01134-4
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发表时间：
2000-06-16
影响因子：
2.5
通讯作者：
Patta, S
中科院分区：
文献类型：
--
作者：
Codina, C;Kraft, R;Patta, S
采用全细胞膜片钳技术研究了髓母细胞瘤细胞系MHH-MED-3和两种髓母细胞瘤活检组织切片和原代培养细胞的电压激活电流和神经递质激活电流。这些制剂表现出相似的电生理特性。所有受试细胞均出现4-氨基吡啶敏感的延迟整流钾电流、γ-氨基丁酸(A)受体介导的氯离子电流和大部分内向整流钾电流。MHH-MED-3细胞的瞬时内向电流主要由低电压激活的T型钙通道和原代培养细胞的河豚毒素敏感的Na+通道携带。根据这些特征，我们得出结论，髓母细胞瘤细胞与发育中的小脑颗粒细胞在未成熟阶段具有共同的生理特征。(C)2000爱思唯尔爱尔兰科学有限公司。保留所有权利。
The whole-cell patch clamp technique was used to characterize voltage- and neurotransmitter-activated currents in the medulloblastoma cell line MHH-MED-3 and cells from tissue slices and primary cultures of two medulloblastoma biopsies. These preparations revealed similar electrophysiological properties. All tested cells displayed 4-aminopyridine-sensitive delayed rectifying K+ currents, gamma-aminobutyric acid(A) receptor-mediated Cl- currents and most of them inward rectifier K+ currents. Transient inward currents were mainly carried by low-voltage activated T-type Ca2+ channels in MHH-MED-3 cells, and tetrodotoxin-sensitive Na+ channels in cells from the primary culture. From these characteristics we conclude that medulloblastoma cells share physiological features with developing cerebellar granule cells at an immature stage. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.