MADS-box transcription factors MADS11 and DAL1 interact to mediate the vegetative-to-reproductive transition in pine
MADS-box transcription factors MADS11 and DAL1 interact to mediate the vegetative-to-reproductive transition in pine
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MADS-box 转录因子 MADS11 和 DAL1 相互作用介导松树从营养到生殖的转变
DOI：
10.1093/plphys/kiab250
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发表时间：
2021-05-29
期刊：
影响因子：
7.4
通讯作者：
Niu, Shi-Hui
中科院分区：
文献类型：
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作者：
Ma, Jing-Jing;Chen, Xi;Niu, Shi-Hui
生殖转换是植物生存和繁殖的重要事件。相对于对被子植物营养体阶段转换的深入了解，对多年生针叶植物营养体阶段转换的研究还很少。为了深入了解针叶树中调控机制的分子基础，我们使用了时间动态转录组分析与样品从7个不同年龄的油松，以确定一个基因模块基本上与衰老相关。研究结果首次表明，油松的相变是一个出乎意料的快速转变，而不是一个缓慢的渐进过程。年龄相关基因模块包含33个转录因子，并且富含属于MADS（MCM 1，AGAMOUS，DEFICIENS，SRF）-盒家族的基因，包括6个S 0 C1样基因和DAL 1和DAL 10。在油松和一个晚结球型白皮松突变体中的表达分析表明PtMADS 11与繁殖能力密切相关。然后，我们证实MADS 11和DAL 1通过物理相互作用协调衰老途径。PtMADS 11和PtDAL 1的过表达部分挽救了拟南芥35 S：：miR 156 A和spl 1，2，3，4，5，6突变体的开花，但只有PtMADS 11可以挽救ft-10突变体的开花，表明PtMADS 11和PtDAL 1在拟南芥开花调控网络中发挥不同的作用。PtMADS 11不能改变soc 1 -1-2的开花表型，表明其在拟南芥中的功能可能与AtSOC 1不同。在这项研究中，我们确定松树中的MADS 11基因是幼年到成年转变的调节介质，其功能与被子植物SOC 1不同。
The reproductive transition is an important event that is crucial for plant survival and reproduction. Relative to the thorough understanding of the vegetative phase transition in angiosperms, a little is known about this process in perennial conifers. To gain insight into the molecular basis of the regulatory mechanism in conifers, we used temporal dynamic transcriptome analysis with samples from seven different ages of Pinus tabuliformis to identify a gene module substantially associated with aging. The results first demonstrated that the phase change in P. tabuliformis occurred as an unexpectedly rapid transition rather than a slow, gradual progression. The age-related gene module contains 33 transcription factors and was enriched in genes that belong to the MADS (MCMl, AGAMOUS, DEFICIENS, SRF)-box family, including six SOC1-like genes and DAL1 and DAL10. Expression analysis in P. tabuliformis and a late-cone-setting P. bungeana mutant showed a tight association between PtMADS11 and reproductive competence. We then confirmed that MADS11 and DAL1 coordinate the aging pathway through physical interaction. Overexpression of PtMADS11 and PtDAL1 partially rescued the flowering of 35S::miR156A and spl1,2,3,4,5,6 mutants in Arabidopsis (Arabidopsis thaliana), but only PtMADS11 could rescue the flowering of the ft-10 mutant, suggesting PtMADS11 and PtDAL1 play different roles in flowering regulatory networks in Arabidopsis. The PtMADS11 could not alter the flowering phenotype of soc1-1-2, indicating it may function differently from AtSOC1 in Arabidopsis. In this study, we identified the MADS11 gene in pine as a regulatory mediator of the juvenile-to-adult transition with functions differentiated from the angiosperm SOC1.