Impact of lime, nitrogen and plant species on bacterial community structure in grassland microcosms
Impact of lime, nitrogen and plant species on bacterial community structure in grassland microcosms
复制标题
DOI：
10.1111/j.1462-2920.2004.00638.x
复制
发表时间：
2004-10-01
影响因子：
5.1
通讯作者：
Clipson, N
中科院分区：
文献类型：
--
作者：
Kennedy, N;Brodie, E;Clipson, N
采用微观方法研究了植物和化学因子对高地草原土壤细菌群落结构的影响。七种多年生植物是天然的、未经改良的典型植物（Nardus stricta、Agrostis capillaris、Festuca ovina和F.在75天的温室实验中，将红草地（Holcus lanatus）和施肥的改良草地（Holcus lanatus、多年生黑麦草（Lolium perenne）和白三叶（Trifolium repens））保持未改良或用石灰、氮或石灰加氮处理。石灰和氮的修正被证明有更大的影响比植物种类的微生物活性，生物量和细菌核糖体数量。石灰增加土壤pH值，微生物活性和生物量，而减少核糖体数量。施氮降低了土壤pH值、微生物活性和核糖体数量。添加石灰和氮有中间效果，这似乎是由石灰比氮驱动。末端限制性片段长度多态性（TRFLP）分析表明，石灰和氮的添加改变了土壤细菌群落结构，而植物种类的影响不大。这些结果进一步证实了多变量冗余分析，并表明土壤石灰和氮状况是更重要的控制细菌群落结构比植物根际效应。
A microcosm-based approach was used to study impacts of plant and chemical factors on the bacterial community structure of an upland acidic grassland soil. Seven perennial plant species typical of both natural, unimproved (Nardus stricta, Agrostis capillaris, Festuca ovina and F. rubra) and fertilized, improved (Holcus lanatus, Lolium perenne and Trifolium repens) grasslands were either left unamended or treated with lime, nitrogen, or lime plus nitrogen in a 75-day glasshouse experiment. Lime and nitrogen amendment were shown to have a greater effect on microbial activity, biomass and bacterial ribotype number than plant species. Liming increased soil pH, microbial activity and biomass, while decreasing ribotype number. Nitrogen addition decreased soil pH, microbial activity and ribotype number. Addition of lime plus nitrogen had intermediate effects, which appeared to be driven more by lime than nitrogen. Terminal restriction fragment length polymorphism (TRFLP) analysis revealed that lime and nitrogen addition altered soil bacterial community structure, while plant species had little effect. These results were further confirmed by multivariate redundancy analysis, and suggest that soil lime and nitrogen status are more important controllers of bacterial community structure than plant rhizosphere effects.