Physical characterization of the chromosomal rearrangements that accompany the transgene insertion in the chakragati mouse mutant.
Physical characterization of the chromosomal rearrangements that accompany the transgene insertion in the chakragati mouse mutant.
复制标题
Chakragati 小鼠突变体中伴随转基因插入的染色体重排的物理特征。
DOI：
10.1006/geno.1997.4975
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Gross,KW
中科院分区：
文献类型：
--
作者：
Smiraglia,DJ;Ratty,AK;Gross,KW
这种转圈的表型是转基因插入突变的结果。在转基因插入杂合子的小鼠中，缺乏表型表明这是由于内源性基因功能的丧失。鉴定该基因的努力导致了之前的一份报告，在野生型小鼠中，转基因D16Ros1和D16Ros2两侧的序列相距10厘米。我们提供的物理作图数据表明，D16Ros1和D16Ros2在小鼠中的接近是由D16Ros2的重复及其插入和D16Ros1上的转基因解释的。我们进一步证明了D16Ros1序列也是重复的，这种重复也是内源D16Ros1座位插入的一部分。
The circling phenotype of thechakragatimouse is a result of a transgenic insertional mutation. The absence of the phenotype in mice heterozygous for the transgene insertion suggests that this is due to a loss of function of an endogenous gene. Efforts to identify this gene have led to a previous report that sequences flanking the transgene,D16Ros1andD16Ros2,map 10 cM apart in wildtype mice. We present here physical mapping data indicating that the proximity ofD16Ros1andD16Ros2in theckrmouse is explained by a duplication ofD16Ros2and its insertion along with the transgene atD16Ros1.We further demonstrate thatD16Ros1sequences are also duplicated and that this duplication is also part of the insertion at the endogenousD16Ros1locus.