Increased synthesis of α-tocopherol, paramylon and tyrosine by Euglena gracilis under conditions of high biomass production
Increased synthesis of α-tocopherol, paramylon and tyrosine by Euglena gracilis under conditions of high biomass production
复制标题
DOI：
10.1111/j.1365-2672.2010.04848.x
复制
发表时间：
2010-12-01
影响因子：
4
通讯作者：
Moreno-Sanchez, R.
中科院分区：
文献类型：
--
作者：
Rodriguez-Zavala, J. S.;Ortiz-Cruz, M. A.;Moreno-Sanchez, R.
目的： 分析暗生长的细小眼虫在细胞高速生长的条件下产生的不同代谢物。 方法和结果： 谷氨酸 (5 g l(-1))、苹果酸 (2 g l(-1)) 和乙醇 (10 ml l(-1)) (GM + EtOH) 的组合；谷氨酸盐(7.15 g·l(-1))和乙醇(10 ml·l(-1))；以苹果酸(8.16 g·l(-1))、葡萄糖(10.6 g·l(-1))和NH4Cl(1.8 g·l(-1))作为碳源和氮源,与谷氨酸和苹果酸(GM)相比,生物量浓度分别提高了5.6、3.7和2.6倍。反过来，LC-MS 鉴定 120 小时后，α-生育酚的产量分别为 3.7 +/- 0.2、2.4 +/- 0.1 和 2 +/- 0.1 mg [g 干重 (DW)](-1)，而在对照培养基 (GM) 中，其产量为 0.72 +/- 0.1 mg (g DW)(-1)。对于裸藻淀粉合成，添加乙醇或葡萄糖会导致更高的产量。通过RP-HPLC测定氨基酸； Tyr（生育酚前体）和 Ala（具有抗氧化活性的氨基酸）是在较高浓度下合成的氨基酸。结论：黑暗生长的 E. gracilis Z 是产生生物技术相关代谢物酪氨酸、α-生育酚和裸藻淀粉的合适来源。研究的意义和影响：通过组合不同的碳源和氮源并通过添加乙醇诱导细胞耐受应激，可以增加生物量的产量，裸藻淀粉、α-生育酚和一些氨基酸。本研究中获得的α-生育酚浓度高于之前报道的眼虫、植物和藻类系统中的其他浓度。这项工作有助于了解不同碳源对 E. gracilis 生物分子合成的影响，并可作为未来工作的基础，以提高该生物体生产具有生物技术重要性的不同代谢物。
Aims: To analyse the production of different metabolites by dark-grown Euglena gracilis under conditions found to render high cell growth.Methods and Results: The combination of glutamate (5 g l(-1)), malate (2 g l(-1)) and ethanol (10 ml l(-1)) (GM + EtOH); glutamate (7.15 g l(-1)) and ethanol (10 ml l(-1)); or malate (8.16 g l(-1)), glucose (10.6 g l(-1)) and NH4Cl (1.8 g l(-1)) as carbon and nitrogen sources, promoted an increase of 5.6, 3.7 and 2.6-fold, respectively, in biomass concentration in comparison with glutamate and malate (GM). In turn, the production of alpha-tocopherol after 120 h identified by LC-MS was 3.7 +/- 0.2, 2.4 +/- 0.1 and 2 +/- 0.1 mg [g dry weight (DW)](-1), respectively, while in the control medium (GM) it was 0.72 +/- 0.1 mg (g DW)(-1). For paramylon synthesis, the addition of EtOH or glucose induced a higher production. Amino acids were assayed by RP-HPLC; Tyr a tocopherol precursor and Ala an amino acid with antioxidant activity were the amino acids synthesized at higher concentration.Conclusions: Dark-grown E. gracilis Z is a suitable source for the generation of the biotechnologically relevant metabolites tyrosine, alpha-tocopherol and paramylon.Significance and Impact of the Study: By combining different carbon and nitrogen sources and inducing a tolerable stress to the cell by adding ethanol, it was possible to increase the production of biomass, paramylon, alpha-tocopherol and some amino acids. The concentrations of alpha-tocopherol achieved in this study are higher than others reported previously for Euglena, plant and algal systems. This work helps to understand the effect of different carbon sources on the synthesis of bio-molecules by E. gracilis and can be used as a basis for future works to improve the production of different metabolites of biotechnological importance by this organism.