Genetic Models of Macrophage Depletion.
Genetic Models of Macrophage Depletion.
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DOI：
10.1007/978-1-4939-7837-3_22
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ren G
中科院分区：
文献类型：
--
作者：
Hua L;Shi J;Shultz LD;Ren G
巨噬细胞是一种异质的先天免疫细胞群，分布在大多数成人组织中。某些来源于产前的组织常驻巨噬细胞，与出生后的单核细胞来源的巨噬细胞一起，作为宿主的清除系统，清除入侵的病原体、恶性细胞、衰老细胞、死细胞、细胞碎片和其他外来物质。巨噬细胞作为单核吞噬细胞系统的关键成员，在产前发育、组织稳态和疾病进展的调控中发挥着重要作用。在过去的二十年里，人们已经做出了相当大的努力来建立小鼠巨噬细胞消融的遗传模型。这些模型支持研究组织特异性巨噬细胞在生理和病理条件下的精确功能。在此，我们概述了目前可用的巨噬细胞体内基因消融的小鼠品系，并讨论了它们各自的优势和局限性。
Macrophages are a heterogeneous population of innate immune cells and are distributed in most adult tissues. Certain tissue resident macrophages with a prenatal origin, together with postnatal monocyte-derived macrophages, serve as the host scavenger system to eliminate invading pathogens, malignant cells, senescent cells, dead cells, cellular debris and other foreign substances. As a key member of the mononuclear phagocyte system, macrophages play essential roles in regulation of prenatal development, tissue homeostasis and disease progression. Over the past two decades, considerable efforts have been made to generate genetic models of macrophage ablation in mice. These models support investigations of the precise functions of tissue specific macrophages under physiological and pathological conditions. Herein, we overview the currently available mouse strains for in vivo genetic ablation of macrophages and discuss their respective advantages and limitations.