Flagella-mediated motility is required for biofilm formation by Erwinia carotovora subsp carotovora
Flagella-mediated motility is required for biofilm formation by Erwinia carotovora subsp carotovora
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DOI：
10.1007/s10327-005-0246-8
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发表时间：
2006-02-01
影响因子：
1.2
通讯作者：
Tsuyumu, Shinji
中科院分区：
文献类型：
--
作者：
Hossain, Md Mijan;Tsuyumu, Shinji
研究鞭毛在胡萝卜软腐欧文氏菌生物膜形成中的作用。carotovora EC 1中，我们使用了一个无鞭毛的，不动的突变体（Δ fliC）和一个有鞭毛的，不动的突变体（Δ motA）。生物膜诱导培养基，其中包含酵母蛋白胨（YP）培养基加上M-63基本培养基的盐，支持生物膜形成的程度比单独的YP或Luria Bertani（LB）培养基。我们证明了Delta fliC和D motA突变体都大大降低了它们在聚氯乙烯（PVC）微量滴定板的威尔斯孔表面上形成生物膜的能力。这两种突变体的不能形成生物膜的PVC表面上进一步证实了相差显微镜。两个aflagellate（三角洲fliC）和鞭毛虫（三角洲motA）nonmotile突变体同样有缺陷的附着到PVC表面。用质子载体羰基氰间氯苯腙（CCCP）处理细菌，抑制这种生物体的运动，大大减少了生物膜的形成。基于这些结果，鞭毛介导的运动可能在E.胡萝卜软腐菌亚种胡萝卜软腐病菌EC 1.
To elucidate the role of flagella in biofilm formation by Erwinia carotovora subsp. carotovora EC1, we used a nonflagellate, nonmotile mutant (Delta fliC) and a flagellate, nonmotile mutant (Delta motA). A biofilm-inducing medium, which contains the yeast peptone (YP) medium plus the salts of M-63 minimal medium, supported biofilm formation to a greater extent than either the YP or Luria Bertani (LB) medium alone. We demonstrated that both the Delta fliC and D motA mutants greatly reduced their ability to form a biofilm on the surface of the wells of polyvinyl chloride (PVC) microtiter plates. The inability of both mutants to form biofilm on the PVC surface was further confirmed with phase-contrast microscopy. Both aflagellate (Delta fliC) and flagellate (Delta motA) nonmotile mutants were equally defective in attachment to the PVC surface. The treatment of bacteria with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP), which inhibits the motility of this organism, reduced greatly the biofilm formation. Based on these results, flagella-mediated motility may play an important role in biofilm formation of E. carotovora subsp. carotovora EC1.