Composition and function of oral microbiota between gingival squamous cell carcinoma and periodontitis
Composition and function of oral microbiota between gingival squamous cell carcinoma and periodontitis
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牙龈鳞状细胞癌和牙周炎之间口腔微生物群的组成和功能
DOI：
10.1016/j.oraloncology.2020.104710
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发表时间：
2020-08-01
期刊：
影响因子：
4.8
通讯作者：
Pan, Yaping
中科院分区：
文献类型：
--
作者：
Li, Yuchao;Tan, Xuexin;Pan, Yaping
目的：牙周炎是口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）的独立危险因素。沿着微生物区系在牙周炎和牙龈鳞状细胞癌（GSCC）中的重要作用，本研究探讨了牙周炎和GSCC中微生物区系的组成和功能。收集唾液、龈下菌斑、舌背、颊粘膜、癌组织和癌旁组织样本。结果：牙周炎病原菌占46%，其中牙周炎病原菌占10%，牙周炎病原菌占10%，牙周炎病原菌占10%。此外，龈下菌斑和唾液中的相互作用分类单位（OTU）分别占38.36%和44.13%。梭杆菌属、消化链球菌属和普雷沃菌属在癌组织中更丰富，而链球菌属、奈瑟氏球菌属和嗜血杆菌属在唾液或软粘膜中更丰富。GSCC舌背和唾液PCoA呈相似的簇状分布。GSCC显示较低的丰富度比牙周炎。在唾液和龈下菌斑中，GSCC中的Atopobium比牙周炎和对照组更普遍，从高到低。结论：牙周炎病原菌在GSCC中的分布较为丰富，GSCC龈下菌斑内脂多糖（LPS）的合成明显高于其他两组。癌组织中牙周致病菌富集，而唾液或软粘膜中牙周健康相关细菌较多。唾液中高水平的Atopobium和LPS生物合成有可能增加牙周炎患者患GSCC的风险，这需要更多的证据来阐明。
Objectives: Previous studies have proved that periodontitis is an independent risk factor of oral squamous cell carcinoma (OSCC) epidemiologically. Along with the important role of microbiota in the cancer process and the specific anatomical position, our study explored the microbial composition and functions in periodontitis and gingival squamous cell carcinoma (GSCC).Materials and Methods: GSCC patients (n = 10), matched periodontitis patients (n = 15), and healthy individuals (n = 15) were recruited. Saliva, subgingival plaque, tongue dorsum, buccal mucosa, cancerous tissue, and paracancerous tissue samples were collected. 16S rDNA amplicon sequencing and functional prediction were applied for the taxonomic analysis.Results: Periodontal pathogens occupied 46% in GSCC. Besides, the mutual operational taxonomy unites (OTU) generated from the subgingival plaque occupied 38.36% and 44.13% from saliva. Fusobacterium, Peptostreptococcus, and Prevotella were more abundant in cancerous tissues, while Streptococcus, Neisseria, and Haemophilus were more enriched in saliva or soft mucosa. PCoA exhibited similar cluster between tongue dorsum and saliva in GSCC. GSCC showed lower richness than periodontitis. In saliva and subgingival plaque, Atopobium was more prevalent in GSCC than periodontitis and controls in descending order. Lipopolysaccharide (LPS) biosynthesis increased in subgingival plaque of GSCC compared with the other two groups.Conclusion: Periodontal pathogens were abundant in GSCC. Cancerous tissues harbor enriched periodontal pathogens while saliva or soft mucosa harbored more periodontal health related bacteria. A high level of Atopobium in saliva and LPS biosynthesis have the potential for increasing the risk of suffering from GSCC in individuals with periodontitis, which needs more evidence to clarify it.