High-throughput optofluidic system for the laser microsurgery of oocytes.
High-throughput optofluidic system for the laser microsurgery of oocytes.
复制标题
用于卵母细胞激光显微手术的高通量光流控系统。
DOI：
10.1117/1.jbo.17.1.015001
复制
发表时间：
2012
影响因子：
3.5
通讯作者：
Berns,MichaelW
中科院分区：
文献类型：
--
作者：
Chandsawangbhuwana,Charlie;Shi,LindaZ;Zhu,Qingyuan;Alliegro,MarkC;Berns,MichaelW
这项研究在显微镜系统中结合了微流控和光学显微消融，允许高通量操作卵母细胞、自动换液和长期观察卵母细胞。该系统的微流控部件将卵母细胞从进气口输送到多个流动通道。在每个通道内，利用外部计算机控制的注射器泵提供的稳定流体，将卵母细胞限制在微流控屏障上。这允许在不干扰卵母细胞位置的情况下轻松地更换培养液。为了消除卵母细胞核仁中直径为5μm的核仁，用固定化的SURF文蛤(Spisula Solidissima)卵母细胞验证了该系统的微流控和激光微束消融能力。跟踪观察卵母细胞极体排出情况。
This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady fluid flow provided by an external computer-controlled syringe pump. This allows for easy media replacement without disturbing the oocyte location. The microfluidic and optical-laser microbeam ablation capabilities of the system were validated using surf clam (Spisula solidissima) oocytes that were immobilized in order to permit ablation of the 5 μm diameternucleolinuswithin the oocyte nucleolus. Oocytes were the followed and assayed for polar body ejection.