The influence of polyunsaturated fatty acids on probiotic growth and adhesion.
The influence of polyunsaturated fatty acids on probiotic growth and adhesion.
复制标题
DOI：
10.1111/j.1574-6968.2001.tb09460.x
复制
发表时间：
2001
影响因子：
2.1
通讯作者：
P. Kankaanpää;Seppo Salminen;E. Isolauri;Y. Lee
中科院分区：
文献类型：
--
作者：
P. Kankaanpää;Seppo Salminen;E. Isolauri;Y. Lee
肠道微生物群和益生菌的建立可以控制肠道的炎症状况。由于多不饱和脂肪酸 (PUFA) 具有抗菌活性，因此可能会阻碍益生菌的作用。我们评估了生长培养基中生理浓度的游离亚油酸、γ-亚麻酸、花生四烯酸、α-亚麻酸和二十二碳六烯酸是否会影响乳杆菌GG（益生菌）、干酪乳杆菌代田（益生菌）和保加利亚乳杆菌（乳制品菌株）的生长和粘附。较高浓度的PUFA（10-40μg PUFA ml（-1））抑制所有测试细菌菌株的生长和粘液粘附，而低浓度的γ-亚麻酸和花生四烯酸（5μg ml（-1））分别促进干酪乳杆菌代田的生长和粘液粘附。 PUFA 还改变了 Caco-2 细胞上的细菌粘附位点。在花生四烯酸存在下生长的 Caco-2 细胞对所有三种细菌菌株的粘附均较少。然而，干酪乳杆菌代田 (L. casei Shirota) 在存在 α-亚麻酸的情况下生长的 Caco-2 细胞上粘附得更好。由于粘膜表面的粘附对于益生菌的健康促进作用至关重要，因此我们的结果表明益生菌在肠道中的作用可能受到膳食多不饱和脂肪酸的调节。
The establishment of the intestinal microflora, and probiotic bacteria, may control the inflammatory conditions in the gut. As polyunsaturated fatty acids (PUFA) possess antimicrobial activities, they may deter the action of probiotics. We assessed whether free linoleic, gamma-linolenic, arachidonic, alpha-linolenic and docosahexaenoic acids at physiological concentrations in the growth media would influence the growth and adhesion of Lactobacillus GG (probiotic), Lactobacillus casei Shirota (probiotic) and Lactobacillus bulgaricus (dairy strain). Higher concentrations of PUFA (10-40 microg PUFA ml(-1)) inhibited growth and mucus adhesion of all tested bacterial strains, whilst growth and mucus adhesion of L. casei Shirota was promoted by low concentrations of gamma-linolenic acid and arachidonic acid (at 5 microg ml(-1)), respectively. PUFA also altered bacterial adhesion sites on Caco-2 cells. Caco-2 cells grown in the presence of arachidonic acid were less adhered to by all three bacterial strains. Yet, L. casei Shirota adhered better on Caco-2 cells grown in the presence of alpha-linolenic acid. As the adhesion to mucosal surfaces is pivotal in health promoting effects by probiotics, our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA.