The effect of adsorbed fibrinogen, fibronectin, von Willebrand factor and vitronectin on the procoagulant state of adherent platelets
The effect of adsorbed fibrinogen, fibronectin, von Willebrand factor and vitronectin on the procoagulant state of adherent platelets
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DOI：
10.1016/s0142-9612(00)00150-2
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发表时间：
2000-11-01
期刊：
影响因子：
14
通讯作者：
Horbett, TA
中科院分区：
文献类型：
--
作者：
Grunkemeier, JM;Tsai, WB;Horbett, TA
促凝（活化）血小板为凝血酶原复合物的组装提供了一个位点，它可以迅速将凝血酶原转化为凝血酶（一种有效的凝块形成诱导剂）。先前，我们报道了血小板与预吸附血浆的表面的粘附使它们成为促凝剂。在本研究中，我们研究了吸附的粘附蛋白（纤维蛋白原（Fg）、纤维连接蛋白（Fn）、血管性血友病因子（vWF）和玻璃体连接蛋白（Vn））对粘附血小板促凝活性的影响。吸附的Fn、vWF和Fg对血小板粘附的促进作用顺序为：Fn < vWF = Fg。然而，这些蛋白促进血小板活化（每粘附血小板产生凝血酶）的顺序如下：Fg < Fn < vWF。用一系列稀释剂吸附正常血浆、血清和缺乏或缺乏血管性血液病因子（de-vWF）、纤维连接蛋白（de-Fn）、玻璃体连接蛋白（de-Vn）或玻璃体连接蛋白和纤维连接蛋白（de-VnFn）的血浆，导致血小板粘附不同，但血小板活化差异不大。然而，用稀释的去vwf血浆预吸附诱导的促凝剂活性低于正常血浆。与Fg预吸附相比，正常血浆预吸附导致了更高水平的血小板活化，这表明血浆蛋白（而不是Fg）的吸附导致了血浆预吸附表面的高水平活化。(C) 2000 Elsevier Science Ltd.版权所有。
Procoagulant (activated) platelets provide a site for assembly of the prothrombinase complex which can rapidly convert prothrombin into thrombin (a potent inducer of clot formation). Previously, we reported that adhesion of platelets to surfaces preadsorbed with blood plasma caused them to become procoagulant. In the present study we investigated the effect of adsorbed adhesion proteins (fibrinogen (Fg), fibronectin (Fn), von Willebrand factor (vWF) and vitronectin (Vn)) on the procoagulant activity of adherent platelets. Adsorbed Fn, vWF and Fg promoted platelet adhesion in the following order: Fn < vWF = Fg. However, these proteins promoted platelet activation (thrombin generation per adherent platelet) in the following order: Fg < Fn < vWF. Adsorption with a series of dilutions of normal plasma, serum, and plasmas deficient in or depleted of von Willebrand factor (de-vWF), fibronectin (de-Fn), vitronectin (de-Vn), or both vitronectin and fibronectin (de-VnFn) resulted in varied platelet adhesion, but little difference in platelet activation. However, preadsorption with dilute de-vWF plasma induced lower procoagulant activity than normal plasma. Preadsorption with normal plasma resulted in higher levels of platelet activation than preadsorption with Fg, suggesting that adsorption of plasma proteins other than Fg caused the high levels of activation observed for plasma preadsorbed surfaces. (C) 2000 Elsevier Science Ltd. All rights reserved.