Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp PCC 6803
Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp PCC 6803
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DOI：
10.1016/s0378-1097(03)00689-x
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发表时间：
2003-10-24
影响因子：
2.1
通讯作者：
Hagemann, M
中科院分区：
文献类型：
--
作者：
Kunert, A;Vinnemeier, J;Hagemann, M
通过将修饰的isiAB启动子片段与gfp融合，并在体内定量测定gfp荧光，分析了Synechocystis sp. PCC 6803中isiAB转录的缺铁依赖性调控。对于假定的Fur box，仅显示对启动子活性的轻微抑制影响。在一个异等位突变体中，富铁条件下isiAB转录的不完全抑制证实了fur在isiAB调控中的作用。然而，在缺铁条件下，isiAB启动子-35盒上游90bp区域是启动子充分活性所必需的。这种模式表明，通过Fur系统显示的抑制机制和未知的激活机制对双启动子进行调控。(C) 2003欧洲微生物学会联合会。Elsevier B.V.版权所有。
The iron deficiency-dependent regulation of isiAB transcription in Synechocystis sp. PCC 6803 was analyzed by fusion of modified isiAB promoter fragments to gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic fur mutant a corresponding incomplete repression of isiAB transcription under iron-replete conditions confirmed the role of Fur in isiAB regulation. However, a 90 bp region upstream of the putative -35 box of the isiAB promoter was essential for full promoter activity under iron-deplete conditions. This pattern indicates a dual promoter regulation by both a repressing mechanism exhibited via the Fur system and an unknown activating mechanism. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.