A PCR-based genetic map for human chromosome 3.
A PCR-based genetic map for human chromosome 3.
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基于 PCR 的人类 3 号染色体遗传图谱。
DOI：
10.1006/geno.1994.1666
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Wilkie,P
中科院分区：
文献类型：
--
作者：
O'Connell,P;Leach,RJ;Rains,D;Taylor,T;Garcia,D;Ballard,L;Holik,P;Weissenbach,J;Sherman,S;Wilkie,P
用聚合酶链式反应(PCR)在CEPH参考家系小组中对125个基于微卫星的简单序列重复序列遗传标记的寡核苷酸引物进行了检测。这些微卫星包括101个二核苷酸重复序列和24个新的四核苷酸重复序列。该标记的平均杂合度为72.4%。用遗传作图软件包LINKING对遗传数据进行分析。这些微卫星标记的一个子集定义了一组跨越271 cM的56个唯一排序的基因座(>；1000：1相对于局部倒位)。另外67个基因座与唯一有序图谱上的标记紧密连锁，但无法进行如此高精度的排序。CMAP将这些标记物定位到可信区间。113个微卫星标记也在3号染色体框架体细胞杂交面板上进行了测试，该面板将该染色体划分为23个细胞遗传学定义的区域，整合了该染色体的遗传和物理图谱。高密度、高杂合度和PCR格式的这组遗传和物理定位的标记将加快新的3号染色体基因的定位和定位克隆。
Oligonucleotide primers for 125 simple sequence repeat microsatellite-based genetic markers have been assayed by polymerase chain reaction (PCR) in the CEPH reference family panel. These microsatellites include 101 dinucleotide repeats as well as 24 new tetranucleotide repeats. The average heterozygosity of this marker set was 72.4%. Genetic data were analyzed with the genetic mapping package LINKAGE. A subset of these microsatellite markers define a set of 56 uniquely ordered loci (>1000:1 against local inversion) that span 271 cM. Sixty-seven additional loci were tightly linked to markers on the uniquely ordered map, but could not be ordered with such high precision. These markers were positioned by CMAP into confidence intervals. One hundred thirteen of the microsatellite markers were also tested on a chromosome 3 framework somatic cell hybrid panel that divides this chromosome into 23 cytogenetically defined regions, integrating the genetic and physical maps of this chromosome. The high density, high heterozygosity, and PCR format of this genetically and physically mapped set of markers will accelerate the mapping and positional cloning of new chromosome 3 genes.