Plasmonic Surfaces for Cell Growth and Retrieval Triggered by Near-Infrared Light.
Plasmonic Surfaces for Cell Growth and Retrieval Triggered by Near-Infrared Light.
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DOI：
10.1002/anie.201509025
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发表时间：
2016-01-18
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影响因子：
--
通讯作者：
Liz-Marzán LM
中科院分区：
文献类型：
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作者：
Giner-Casares JJ;Henriksen-Lacey M;García I;Liz-Marzán LM
在组织工程和再生医学中，需要有效地分离细胞以避免损伤的方法。我们介绍了一种自下而上的方法来构建等离子体基板，使用胶束嵌段共聚物纳米光刻来生成二维阵列的Au种子，随后化学生长导致各向异性纳米颗粒。由此产生的等离子体基板显示出一个宽的等离子体激元带，覆盖了可见光和近红外(NIR)光谱范围的很大一部分。人和小鼠细胞均在该基质上成功生长。用环状精氨酸甘氨酰天冬氨酸(c-RGD)肽对血浆底物进行简单的功能化处理，我们就可以调整富含整合素的人脐静脉内皮细胞(HUVEC)的形态。随后用近红外激光照射导致细胞高效分离，并用四甲基偶氮唑盐比色法证实细胞活性。因此，我们建议使用这种等离子体底物进行细胞生长，并使用远程近红外辐射控制分离，作为生物医学应用中细胞培养的一般方法。
Methods for efficient detachment of cells avoiding damage are required in tissue engineering and regenerative medicine. We introduce a bottom–up approach to build plasmonic substrates using micellar block copolymer nanolithography to generate a 2D array of Au seeds, followed by chemical growth leading to anisotropic nanoparticles. The resulting plasmonic substrates show a broad plasmon band covering a wide part of the visible and near‐infrared (NIR) spectral ranges. Both human and murine cells were successfully grown on the substrates. A simple functionalization step of the plasmonic substrates with the cyclic arginylglycylaspartic acid (c‐RGD) peptide allowed us to tune the morphology of integrin‐rich human umbilical vein endothelial cells (HUVEC). Subsequent irradiation with a NIR laser led to highly efficient detachment of the cells with cell viability confirmed using the MTT assay. We thus propose the use of such plasmonic substrates for cell growth and controlled detachment using remote near‐IR irradiation, as a general method for cell culture in biomedical applications.