Ratiometric fluorescence probes based on a Michael acceptor type of coumarin and their application for the multichannel imaging of in vivo glutathione
Ratiometric fluorescence probes based on a Michael acceptor type of coumarin and their application for the multichannel imaging of in vivo glutathione
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DOI：
10.1039/c4ra01933d
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发表时间：
2014-01-01
期刊：
影响因子：
3.9
通讯作者：
Kim, Hae-Jo
中科院分区：
文献类型：
--
作者：
Kim, Gun-Joong;Yoon, Doo-Ha;Kim, Hae-Jo
开发了一系列基于香豆素部分的迈克尔受体作为荧光探针，用于体内谷胱甘肽的比例检测。通过生物硫醇的迈克尔加成，α,β-不饱和迈克尔受体转化为非共轭分子。所得探针的紫外可见光谱和荧光光谱揭示了特征比率响应，该响应成功应用于体内谷胱甘肽的多通道成像。
A series of Michael acceptors based on a coumarin moiety, were developed as fluorescent probes for ratiometric detection of in vivo glutathione. The alpha,beta-unsaturated Michael acceptors were transformed into non-conjugated molecules through the Michael addition of biothiols. The resulting UV-vis and fluorescence spectra of the probes revealed characteristic ratiometric responses, which were successfully applied for the multichannel imaging of in vivo glutathione.