A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation
A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation
复制标题
DOI：
10.1046/j.1472-765x.2003.01394.x
复制
发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
François, JM
中科院分区：
文献类型：
--
作者：
Aguilar-Uscanga, B;François, JM
目的：测量不同生长条件下酿酒酵母细胞壁的多糖组成，并与细胞壁结构进行比较。方法和结果：采用化学和酶法测定酵母细胞壁的β-1,3-葡聚糖和1,6-葡聚糖、甘露聚糖和几丁质的水平，并通过对酶解酶裂解作用的敏感性定性评估细胞壁的结构/抵抗力。研究发现，细胞壁的干质量和多糖含量可随碳源、氮限制、pH、温度和通气的性质以及细胞培养模式（摇瓶与控制发酵罐）的变化而变化超过50%。虽然β-葡聚糖或甘露聚糖水平与整个酵母细胞对酶解酶的敏感性之间没有发现明显的相关性，但β-1,6-葡聚糖水平的增加（尽管相对于所研究的生长条件而言是适度的）以及几丁质的增加，与酵母细胞对酶解酶裂解作用的敏感性降低有关。该研究的意义和影响：我们的结果表明，细胞壁结构仅由细胞壁之间的交联决定聚合物，指出了β-1,6-葡聚糖在此过程中的作用。因此，这项研究强化了这样的观点：参与这些交联反应的酶是抗真菌药物的潜在靶标。
Aim: The polysaccharide composition of the Saccharomyces cerevisiae cell wall was measured under various growth conditions and was compared with the cell wall structure.Methods and Results: Chemical and enzymatic methods were used to determine levels of beta-1,3-glucan and 1,6-glucan, mannan and chitin of the yeast cell wall, whereas the structure/resistance of the wall was qualitatively assessed by the sensibility to the lytic action by zymolyase. It was found that the dry mass and polysaccharides content of the cell wall could vary by more than 50% with the nature of the carbon source, nitrogen limitation, pH, temperature and aeration, and with the mode of cell cultivation (shake flasks vs controlled fermentors). While no obvious correlation could be found between beta-glucan or mannan levels and the susceptibility of whole yeast cells to zymolyase, increase of beta-1,6-glucan levels, albeit modest with respect to the growth conditions investigated, and to a lesser extent that of chitin, was associated with decreased sensitivity of yeast cells to the lytic action by zymolyase.Significance and Impact of the Study: Our results indicate that the cell wall structure is merely determined by cross-linking between cell wall polymers, pointed out the role of beta-1,6-glucan in this process. Hence, this study reinforces the idea that enzymes involved in these cross-linking reactions are potential targets for antifungal drugs.