Genetic characterization of HIV-1 epidemic in Anhui Province, China
Genetic characterization of HIV-1 epidemic in Anhui Province, China
复制标题
中国安徽省HIV-1流行的遗传特征
DOI：
10.1186/s12985-020-1281-y
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发表时间：
2020-02-03
期刊：
影响因子：
4.8
通讯作者：
Li, Lin
中科院分区：
文献类型：
--
作者：
Zhang, Dong;Wu, Jianjun;Li, Lin
背景安徽省中国正面临着严重的艾滋病疫情，新诊断病例越来越多。对安徽省15个区新报告的HIV感染者进行了登记和访谈。从血样分离的血浆中提取病毒总RNA。我们扩增并测序了1062 碱基的HIVpol片段。这些序列用于确定HIV亚型和是否存在耐药突变。传输网络的构建是为了探索可能的关系。结果共获得486例患者的部分Poll基因序列，并将其提交到Stanford HIV耐药性数据库网站。结果表明，男男性接触者是最主要的传播途径(253例，52.06%)，其次为异性传播(210例，43.21%)和血液传播(1例，0.21%)。亚型包括CRF01_AE(226，46.50%)，CRF07_BC(151，31.07%)，B亚型(28，5.76%)，CRF08_BC(20，4.12%)，CRF55_01B(15，3.09%)，CRF68_01B(7，1.44%)，CRF67_01B(3，0.62%)，CRF57_BC(2，0.41%)，CRF59_01B(2，0.41%)，CRF79_0107(2，0.41%)，0.41%)、CRF64_BC(1例，0.21%)和循环重组形式(27例，5.55%)。根据CRF01AE和CRF07BC序列确定了4个含有高危个体(≥4级)的传播亚网，其中包括两个由平均年龄分别为67.9岁和61.5 岁的老年人构成的CRF01_AE传播亚网。感染最可能通过异性传播，而另外两个CRF07_BC传播子网络主要由平均年龄为31.73岁和34.15岁的MSM组成。HIV传播耐药率为3.09%。结论安徽省HIV多亚型同时流行，提示有必要密切监测当地HIV流行情况。此外，老年人经常参与其中，他们主张在这一特定人群中进行行为干预，而不是MSM风险群体。
BackgroundAnhui Province in China is facing a severe HIV epidemic with an increasing number of newly diagnosed cases.MethodsIn this study, HIV genetic characteristics in the province were investigated. Newly reported HIV-positive individuals from 15 districts of Anhui Province were enrolled and interviewed. Total viral RNA was extracted from plasma isolated from blood samples. We amplified and sequenced an HIVpolfragment of the 1062 bp. The sequences were used for determination of HIV subtypes and the presence of drug resistance mutations. Transmission networks were constructed to explore possible relationships. And all of assembled partial pol genes were submitted to the Stanford HIV Drug Resistance Database website to find the transmitted drug resistance.ResultsPartialpolgene sequences were obtained from 486 cases. The results showed that MSM was the most dominant transmission route (253, 52.06%), followed by heterosexual transmission (210, 43.21%) and blood-borne transmission (1, 0.21%). Many subtypes were identified, including CRF01_AE (226, 46.50%), CRF07_BC (151, 31.07%), subtype B (28, 5.76%), CRF08_BC (20, 4.12%), CRF55_01B (15, 3.09%), CRF68_01B (7, 1.44%), CRF67_01B (3, 0.62%), CRF57_BC (2, 0.41%), CRF59_01B (2, 0.41%), CRF79_0107 (2, 0.41%), subtype C (2, 0.41%), CRF64_BC (1, 0.21%), and circulating recombinant forms (URFs) (27, 5.55%). Four transmission subnetworks containing high transmission risk individuals (with degree ≥4) were identified based on CRF01_AE and CRF07_BC sequences, including two CRF01_AE transmission subnetworks constituted by elderly people with average ages of 67.9 and 61.5 years. Infection occurred most likely through heterosexual transmission, while the other two CRF07_BC transmission subnetworks consist mainly of MSMs with average ages of 31.73 and 34.15. The level of HIV-transmitted drug resistance is 3.09%.ConclusionsThe simultaneous spread of multiple HIV subtypes in Anhui province underscores that close surveillance of the local HIV epidemic is necessary. Furthermore, the elderly people were frequently involved, arguing for behaviour intervention in this specific population besides the MSM risk group.