Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation
Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation
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DOI：
10.1186/1476-9255-5-1
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发表时间：
2008-02-08
影响因子：
5.1
通讯作者：
Hartle, Diane K.
中科院分区：
文献类型：
--
作者：
Bralley, Eve E.;Greenspan, Phillip;Hartle, Diane K.
工作背景：本研究测试了表征的虎杖（Polygonum cuspidatum）提取物（PCE）响应于局部施用12-O-十四酰基佛波醇-13-乙酸酯（TPA）抑制小鼠耳部炎症的能力。在TPA给药（2 μ g/耳）后30分钟，将1 PCE以0.075、0.15、0.3、1.25和2.5 mg/耳施用于雌性Swiss小鼠（n = 8）的双耳。为了比较，用TPA和1）单独的媒介物（50%乙醇）、2）吲哚美辛（0.5mg/耳）或3）反式白藜芦醇（0.62mg/耳）处理另外3个组。在TPA给药前和TPA给药后4小时和24小时测量耳厚度，以评估耳水肿。在24小时收集耳穿孔活组织检查并称重作为水肿的第二指标。髓过氧化物酶活性测定，在每个耳朵打孔活检，以评估中性粒细胞infiltration.Results：PCE治疗在所有剂量显着减少耳水肿相比，TPA控制。PCE反应呈剂量依赖性，2.5 mg PCE对所有炎症标志物的抑制作用显著高于吲哚美辛（0.5 mg）。在PCE剂量>= 1.25 mg/耳时，MPO活性受到抑制。Trans-resveratrol inhibitors in comparable doses.Conclusion：PCE抑制TPA治疗的小鼠耳局部炎症模型中的水肿和中性粒细胞浸润的发展。
Background: This study tested the ability of a characterized extract of Polygonum cuspidatum (PCE) to inhibit mouse ear inflammation in response to topical application of 12-O-tetradecanoylphorbol-13-acetate (TPA).Methods: A 50% (wt:vol) ethanolic solution of commercial 200: 1 PCE was applied to both ears of female Swiss mice (n = 8) at 0.075, 0.15, 0.3, 1.25 and 2.5 mg/ear 30 min after TPA administration (2 mu g/ear). For comparison, 3 other groups were treated with TPA and either 1) the vehicle (50% ethanol) alone, 2) indomethacin (0.5 mg/ear), or 3) trans-resveratrol (0.62 mg/ear). Ear thickness was measured before TPA and at 4 and 24 h post-TPA administration to assess ear edema. Ear punch biopsies were collected at 24 h and weighed as a second index of edema. Myeloperoxidase activity was measured in each ear punch biopsy to assess neutrophil infiltration.Results: PCE treatment at all doses significantly reduced ear edema compared to the TPA control. The PCE response was dose-dependent and 2.5 mg PCE significantly inhibited all markers of inflammation to a greater extent than indomethacin (0.5 mg). MPO activity was inhibited at PCE doses >= 1.25 mg/ear. Trans-resveratrol inhibited inflammation at comparable doses.Conclusion: PCE inhibits development of edema and neutrophil infiltration in the TPA-treated mouse ear model of topical inflammation.