Development of cultivated mucosal epithelial sheet transplantation for ocular surface reconstruction
Development of cultivated mucosal epithelial sheet transplantation for ocular surface reconstruction
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DOI：
10.1111/j.1525-1594.2004.07319.x
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发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Nakamura, T
中科院分区：
文献类型：
--
作者：
Kinoshita, S;Nakamura, T
组织工程再生医学是基于利用成体干细胞产生生物替代品并改善组织功能的新兴医学领域。这些目标的实现取决于两个重要因素：具有高增殖性和分化性的干细胞，以及支持它们的基质。为了重建严重眼表疾病患者的眼表，我们决定研究人羊膜作为上皮载体的可行性，并发现裸露的羊膜是最合适的基质。为了开发使用裸露羊膜作为载体的手术治疗方法，我们建立了组织工程系统，用于制造可移植的角膜或口腔粘膜上皮细胞上皮片，用于眼表重建。
Regenerative medicine via tissue engineering is a newly developed medical field based on the use of somatic stem cells to generate biological substitutes and improve tissue functions. The achievement of these objectives depends on two important factors: stem cells, which are high in proliferability and differentiability, and the substrates that support them. In order to reconstruct the ocular surface in patients with severe ocular surface diseases, we decided to investigate the feasibility of human amniotic membrane as an epithelial carrier, and found that denuded amniotic membrane was the most appropriate substrate for this purpose. To develop the surgical treatment using denuded amniotic membrane as a carrier, we established the tissue engineering system for making transplantable epithelial sheets of either corneal or oral mucosal epithelial cells for ocular surface reconstruction.