Translation repression by an RNA polymerase elongation complex.
Translation repression by an RNA polymerase elongation complex.
复制标题
RNA 聚合酶延伸复合物的翻译抑制。
DOI：
10.1111/j.1365-2958.2004.04170.x
复制
发表时间：
2004
影响因子：
3.6
通讯作者：
Court,DonaldL
中科院分区：
文献类型：
--
作者：
Wilson,HelenR;Zhou,Jian-Guang;Yu,Daiguan;Court,DonaldL
Bacteriophage λ N and bacterial Nus proteins together with a unique site NUT in the leader of the early viralNgene transcript bind RNA polymerase (RNAP) and form a highly processive antitermination complex; N bound at NUT also repressesNtranslation. In this study, we investigate whether N and NUT cause N translation repression as part of the antitermination complex by testing conditions that inhibit the formation of the N‐modified transcription complex for their effect on N‐mediated translation repression. We show thatnusandnutmutations that in combination destabilize multiple interactions in the antitermination complex prevent N‐mediated translation repression. Likewise, transcription of thenut‐Nregion by T7 RNAP, which does not lead to the assembly of an effective antitermination complex when N is supplied, eliminates translation repression. We also demonstrate that a unique mutant β subunit of RNAP reduces N‐mediated translation repression, and that overexpression of transcription factor NusA suppresses this defect. We conclude that the N‐modified RNAP transcription complex is necessary to repressNtranslation.
