Equilibrium constants for the binding of aluminum to human serum transferrin
Equilibrium constants for the binding of aluminum to human serum transferrin
复制标题
铝与人血清转铁蛋白结合的平衡常数
DOI：
10.1021/ic00326a024
复制
发表时间：
1990
影响因子：
4.6
通讯作者：
J. Sheldon
中科院分区：
文献类型：
--
作者：
W. R. Harris;J. Sheldon
在0.10M N-(2-hydroxyethyl)piperazine-N‘-ethanesulfonic酸和5 mM碳酸氢钠溶液中，用差示紫外分光光度法研究了pH为7.4时铝与人血清转铁蛋白的结合。铝结合产生240和288 nm的峰，这是转铁蛋白特定的金属结合位点结合的特征。240 nm处较强的峰被用来测定两个宏观铝结合常数
The binding of aluminium to human serum transferrin in 0.10 M N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid and 5 mM sodium bicarbonate at pH 7.4 has been studied by difference ultraviolet spectrophotometry. Aluminium binding produces peaks at 240 and 288 nm that are characteristic of binding at the transferrin specific metal binding sites. The more intense peak at 240 nm has been used to determine two macroscopic aluminium binding constants