In Cellullo and ex Vivo Availability of a Yellowish-Green-Light-Controllable NO Releaser
In Cellullo and ex Vivo Availability of a Yellowish-Green-Light-Controllable NO Releaser
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DOI：
10.1248/cpb.c19-00112
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发表时间：
2019-06-01
影响因子：
1.7
通讯作者：
Nakagawa, Hidehiko
中科院分区：
文献类型：
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作者：
Leda, Naoya;Hotta, Yuji;Nakagawa, Hidehiko
时空可控的一氧化氮(NO)释放剂是研究NO生物活性的非常有吸引力的化学工具，NO参与血管扩张、神经传递和免疫反应的调节。我们前期开发了一种易于合成、黄绿光可控的NO释放剂NO-Rosa5，并表征了其光氧化还原反应机理。在这里，我们的目的是建立NO-Rosa5在细胞内和体外实验中的生物学适用性。我们成功地证明了黄绿光在体外控制HEK293T细胞中NO的释放，以及在体外系统中光操纵大鼠主动脉对NO的反应。此外，四氮唑盐细胞活力实验表明，NO-Rosa5的毒性低于先前报道的蓝光可控NO释放剂NOBL-1。总体而言，我们的结果表明，NO-Rosa5是一种生物相容的、光可控的NO释放剂，毒性低，具有广阔的应用前景。
Spatiotemporally controllable nitric oxide (NO) releasers are very attractive chemical tools for investigating the biological activities of NO, which is involved in the regulation of vasodilation, neurotransmission, and immune responses. We previously developed an easily synthesized, yellowish-green-light-controllable NO releaser, NO-Rosa5, and characterized its photoredox reaction mechanism. Here, we aimed to establish the biological applicability of NO-Rosa5 for in cellullo and ex vivo experiments. We successfully demonstrated yellowish-green-light-controlled NO release in HEK293T cells in vitro, as well as photomanipulation of the rat aorta response to NO in an ex vivo system. Furthermore, NO-Rosa5 showed lower toxicity than NOBL-1, a previously reported blue-light-controllable NO releaser, as determined by tetrazolium salt cell viability assay. Overall, our results indicate that NO-Rosa5 is a biocompatible, photocontrollable NO releaser with low toxicity and potentially broad applicability.