Autonomous and nonautonomous functions for Hox/Pbx in branchiomotor neuron development
Autonomous and nonautonomous functions for Hox/Pbx in branchiomotor neuron development
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DOI：
10.1016/s0012-1606(02)00018-0
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发表时间：
2003-01-15
影响因子：
2.7
通讯作者：
Moens, CB
中科院分区：
文献类型：
--
作者：
Cooper, KL;Leisenring, WM;Moens, CB
脊椎动物鳃裂神经元的组织模式与发育中的后脑的节段或菱形节相对应，并且具有与它们沿沿着前/后轴的位置相关的身份和行为。这些神经元在后脑中经历特征性的迁移，并从刻板的出口点投射。我们发现，Lazarus/pbx 4，它编码一个必不可少的Hox DNA结合的合作伙伴在斑马鱼，需要面部（第七脑神经）运动神经元迁移和三叉神经（第五脑神经）运动轴突的轴突寻路。我们发现，lzr/pbx 4是所需的Hox paradox组1和2的功能，这表明Pbx与这些蛋白质相互作用。与此相一致的是，lzr/pbx 4与hoxb 1a在遗传上相互作用来控制面部运动神经元的迁移。使用遗传镶嵌分析，我们表明，lzr/pbx 4和hoxb 1a主要需要细胞自主内的面部运动神经元，然而，一个微妙的非细胞自主效应的分析表明，面部运动神经元迁移促进迁移神经元之间的相互作用。同时，lzr/pbx 4需要非细胞自主地控制三叉神经运动轴突的寻路。因此，Pbx/Hox可以细胞自主和非细胞自主地发挥作用，以指导后脑运动神经元行为的不同方面。(C)2003 Elsevier Science（美国）。All rights reserved.
The vertebrate branchiomotor neurons are organized in a pattern that corresponds with the segments, or rhombomeres, of the developing hindbrain and have identities and behaviors associated with their position along the anterior/posterior axis. These neurons undergo characteristic migrations in the hindbrain and project from stereotyped exit points. We show that lazarus/pbx4, which encodes an essential Hox DNA-binding partner in zebrafish, is required for facial (VIIth cranial nerve) motor neuron migration and for axon pathfinding of trigeminal (Vth cranial nerve) motor axons. We show that lzr/pbx4 is required for Hox paralog group 1 and 2 function, suggesting that Pbx interacts with these proteins. Consistent with this, lzr/pbx4 interacts genetically with hoxb1a to control facial motor neuron migration. Using genetic mosaic analysis, we show that lzr/pbx4 and hoxb1a are primarily required cell-autonomously within the facial motor neurons; however, analysis of a subtle non-cell-autonomous effect indicates that facial motor neuron migration is promoted by interactions amongst the migrating neurons. At the same time, lzr/pbx4 is required non-cell-autonomously to control the pathfinding of trigeminal motor axons. Thus, Pbx/Hox can function both cell-autonomously and non-cell-autonomously to direct different aspects of hindbrain motor neuron behavior. (C) 2003 Elsevier Science (USA). All rights reserved.