The functions of Klarsicht and nuclear lamin in developmentally regulated nuclear migrations of photoreceptor cells in the Drosophila eye
The functions of Klarsicht and nuclear lamin in developmentally regulated nuclear migrations of photoreceptor cells in the Drosophila eye
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DOI：
10.1091/mbc.e03-06-0374
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发表时间：
2004-02-01
影响因子：
3.3
通讯作者：
Fischer, JA
中科院分区：
文献类型：
--
作者：
Patterson, K;Molofsky, AB;Fischer, JA
果蝇眼睛中的感光细胞核经历发育调节的迁移。已知核迁移需要核周蛋白Klarsicht，但Klarsicht的功能一直不清楚。在这里，我们表明，Klarsicht是连接微管组织中心（MTCC）的细胞核。此外，在klarsicht相互作用基因的遗传筛选中，我们鉴定了编码核纤层蛋白的Lam Dm（o）。我们发现，像Klarsicht，核纤层蛋白所需的感光细胞核迁移和核附着的MTCC。此外，Klarsicht的核周定位需要核纤层蛋白。我们建议，核迁移需要通过微管，Klarsicht，和核纤层蛋白之间的相互作用的MTOC的联系。Klarsicht/核纤层蛋白相互作用为理解人类核纤层蛋白病的机制基础提供了一个框架。
Photoreceptor nuclei in the Drosophila eye undergo developmentally regulated migrations. Nuclear migration is known to require the perinuclear protein Klarsicht, but the function of Klarsicht has been obscure. Here, we show that Klarsicht is required for connecting the microtubule organizing center (MTCC) to the nucleus. In addition, in a genetic screen for klarsicht-interacting genes, we identified Lam Dm(o), which encodes nuclear lamin. We find that, like Klarsicht, lamin is required for photoreceptor nuclear migration and for nuclear attachment to the MTCC. Moreover, perinuclear localization of Klarsicht requires lamin. We propose that nuclear migration requires linkage of the MTOC to the nucleus through an interaction between microtubules, Klarsicht, and lamin. The Klarsicht/lamin interaction provides a framework for understanding the mechanistic basis of human laminopathies.