Multiplexed detection and identification of respiratory pathogens using the NxTAG® respiratory pathogen panel
Multiplexed detection and identification of respiratory pathogens using the NxTAG® respiratory pathogen panel
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DOI：
10.1016/j.ymeth.2019.01.005
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发表时间：
2019-04-01
期刊：
影响因子：
4.8
通讯作者：
Juretschko, Stefan
中科院分区：
文献类型：
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作者：
Gonsalves, Sarah;Mahony, James;Juretschko, Stefan
Luminex（R）NxTAG（R）呼吸道病原体检测试剂盒（NxTAG RPP）是一种经IVD批准的检测试剂盒，用于同时检测和鉴定18种呼吸道病毒和2种（或3种美国以外的病毒）鼻咽拭子中的非典型细菌病原体。其可扩展性允许在一个批次中同时测试多达96个样品。将使用easyMAG提取器从200 μ L原始样本中提取的核酸直接添加到预铺板的冻干珠试剂（LBR）中，其中使用单一循环程序在密封的反应孔内进行多重RT-PCR和与MagPlex-TAG（TM）微球的杂交。在MAGPIX（R）仪器上进行数据采集，该仪器在从热循环仪转移测定板之后直接从密封孔读取并分类反应产物。NxTAG是Luminex开发的基于珠的核酸化学的最新创新。在此，我们提供了详细的试验方案，并提供了描述NxTAG RPP临床和分析性能特征的数据。
The Luminex (R) NxTAG (R) Respiratory Pathogen Panel (NxTAG RPP) is an IVD-cleared assay for the simultaneous detection and identification of nucleic acids from 18 respiratory viruses and 2 (or 3 outside of the U.S.) atypical bacterial pathogens in nasopharyngeal swabs. Its scalability allows concurrent testing of up to 96 samples in a single batch. Nucleic acid extracted from 200 mu L of raw specimen using the easyMAG (R) extractor is added directly to pre-plated, lyophilized bead reagents (LBRs), where multiplexed RT-PCR and hybridization to MagPlex-TAG (TM) microspheres occurs within a sealed reaction well using a single cycling program. Data acquisition is done on the MAGPIX (R) instrument which reads and sorts the reaction products directly from the sealed well following transfer of the assay plate from the thermal cycler. NxTAG is the newest innovation in bead-based nucleic acid chemistry developed by Luminex. Here we provide the detailed assay protocol and present data which describe the clinical and analytical performance characteristics of NxTAG RPP.