G-PROTEIN AND DIACYLGLYCEROL REGULATE METAMORPHOSIS OF PLANKTONIC MOLLUSCAN LARVAE
G-PROTEIN AND DIACYLGLYCEROL REGULATE METAMORPHOSIS OF PLANKTONIC MOLLUSCAN LARVAE
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DOI：
10.1073/pnas.84.7.1867
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
MORSE, DE
中科院分区：
文献类型：
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作者：
BAXTER, G;MORSE, DE
调节鸟嘌呤核苷酸结合蛋白（G蛋白）激活霍乱毒素和GTP类似物5-鸟苷酰亚胺磷酸，第二信使甘油二酯，和某些二氨基酸都有利于（放大）的解决和变质反应的红鲍（海洋软体动物）的寄生幼虫形态发生的化学刺激。相比之下，G蛋白抑制类似物鸟苷5“-O-[β-硫代]二磷酸抑制L-α，.贝塔二氨基丙酸，但不阻断二酰基甘油的促进作用。二酰基甘油、霍乱毒素和鸟嘌呤核苷酸类似物单独既不诱导幼虫的定居和变态，也不抑制γ-谷氨酰胺对变态的诱导。氨基丁酸因此，这些结果建立了独立的监管和诱导途径控制幼虫变态反应的两类外源性化学信号从环境中的存在。调节途径，独立运作，通过G蛋白-甘油二酯级联显然是由促进二氨基酸在水柱中控制，可以放大幼虫变态诱导剂的反应。这一机制可能对幼虫识别和选择变态的有利生境具有适应意义。类似的调节途径，基于外源性控制的G蛋白-二酰基甘油级联反应，可能会在其他感觉和发育系统中对刺激的反应进行管理。
The regulatory guanine nucleotide binding protein (G protein) activators cholera toxin and the GTP analog 5-guanylyl imidophosphate, the second messenger diacylglycerol, and certain diamino acids all facilitate (amplify) the settlement and metamorphic responses of planktonic larvae of Haliotis rufescens (marine mollusc) to morphogenetic chemical stimuli. In contrast, the G protein-inhibiting analog guanosine 5''-O-[.beta.-thio]diphosphate inhibits facilitation by L-.alpha.,.beta.-diaminopropionic acid but does not block facilitation by diacylglycerol. Diacylglycerol, cholera toxin, and the guanine nucleotide analogs alone neither induce the settlement and metamorphosis of the larvae nor do they inhibit induction of metamorphosis by .gamma.-aminobutyric acid. These results thus establish the existence of separate regulatory and inductive pathways controlling larval metamorphosis in response to two classes of exogenous chemical signals from the environment. The regulatory pathway, operating independently through a G protein-diacylglycerol cascade apparently controlled by facilitating diamino acids in the water column, can amplify the larval responsiveness to inducers of metamorphosis. This mechanism may have adaptive significance in the recognition and selection of favorable habitats for metamorphosis of the larvae. Similar regulatory pathways, based on exogenous control of a G protein-diaclyglycerol cascade, may govern responsiveness to stimuli in other sensory and developmental systems.