QUANTITATION OF PREPROENKEPHALIN MESSENGER-RNA LEVELS IN BRAIN-REGIONS FROM MALE FISCHER RATS FOLLOWING CHRONIC COCAINE TREATMENT USING A RECENTLY DEVELOPED SOLUTION HYBRIDIZATION ASSAY
QUANTITATION OF PREPROENKEPHALIN MESSENGER-RNA LEVELS IN BRAIN-REGIONS FROM MALE FISCHER RATS FOLLOWING CHRONIC COCAINE TREATMENT USING A RECENTLY DEVELOPED SOLUTION HYBRIDIZATION ASSAY
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DOI：
10.1016/0169-328x(92)90178-e
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发表时间：
1992-07-01
期刊：
影响因子：
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通讯作者：
KREEK, MJ
中科院分区：
文献类型：
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作者：
BRANCH, AD;UNTERWALD, EM;KREEK, MJ
用定量溶液杂交法测定了连续14天每天三次腹腔注射可卡因（10或30 mg/kg/d）或生理盐水的大鼠8个脑区提取物中前脑啡肽原mRNA（PPenk mRNA）的皮克量和总RNA的微克量。最后一次注射后30分钟处死年轻成年雄性Fischer大鼠。在纹状体提取物中发现PPenk mRNA的最高密度（pg PPenk mRNA/μ g总细胞RNA）（对于11只盐水处理的大鼠为34.08 +/-1.79 pg/μ g），其次是丘脑核提取物（10.08 +/-0.81 pg/μ g）和下丘脑提取物（2.99 +/-0.31 pg/μ g）。额叶皮质（1.78 +/- 0.24 pg/μ g）、垂体（1.39 +/- 0.08 pg/μ g）、中央灰质（1.31 +/- 0.16 pg/μ g）和小脑（1.24 +/- 0.09 pg/μ g）的提取物具有中等值。海马提取物（0.53 +/- 0.03 pg/μ g）的密度最低。在研究的任何脑区中，未发现治疗组之间存在显著差异。因此，在该方案中给予的慢性可卡因治疗没有改变编码脑啡肽原的基因的表达。
Quantitative solution hybridization assays were used to determine the picogram amounts of preproenkephalin mRNA (PPenk mRNA) and the microgram quantities of total rat RNA in extracts of eight brain regions from rats which had received three daily intraperitoneal injections of cocaine (10 or 30 mg/kg/day) or saline for 14 days. The young adult male Fischer rats were sacrificed 30 min after the final injection. The highest density of PPenk mRNA (pg PPenk mRNA/mu-g total cellular RNA) was found in extracts of striatum (34.08 +/- 1.79 pg/mu-g for 11 saline-treated rats), followed by extracts of nucleus accumbens (10.08 +/- 0.81 pg/mu-g), and extracts of hypothalamus (2.99 +/- 0.31 pg/mu-g). Extracts of frontal cortex (1.78 +/- 0.24 pg/mu-g), pituitary (1.39 +/- 0.08 pg/mu-g), central grey (1.31 +/- 0.16 pg/mu-g), and cerebellum (1.24 +/- 0.09 pg/mu-g) had intermediate values. Extracts of hippocampus (0.53 +/- 0.03 pg/mu-g) had the lowest density. No significant differences were found among the treatment groups in any brain area investigated. Therefore, chronic cocaine treatment as administered in this protocol did not alter expression of the gene encoding proenkephalin.