Propagation pattern of entorhinal cortex subfields to the dentate gyrus in the guinea-pig: an electrophysiological study.
Propagation pattern of entorhinal cortex subfields to the dentate gyrus in the guinea-pig: an electrophysiological study.
复制标题
豚鼠内嗅皮层子域到齿状回的传播模式：一项电生理学研究。
DOI：
10.1016/s0306-4522(03)00551-7
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发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
deCurtis,M
中科院分区：
文献类型：
--
作者：
Uva,L;deCurtis,M
解剖学研究表明，位于内嗅皮层（EC）的内侧和外侧方面的浅层神经元的项目的齿状回（DG）和海马CA1区的颞叶和隔部，分别。在本研究中，我们研究了电生理技术的传播模式，不同的EC子字段的在离体豚鼠脑DG。从DG的不同部分的层流场电位分布记录与多通道硅探针直接刺激后，在直接视觉控制下的不同位置进行的同侧EC表面。电流源密度分析的层状配置文件表明，i）刺激的喙内侧EC诱导的单突触反应，专门在DG的颞极，ii）刺激的外侧和尾部的EC确定了一个弥漫性的单突触激活的中间和隔DG。EC的区域，项目的DG在豚鼠的不同部门不相关的EC子字段的基础上确定的cytoarchitectonic标准。EC诱发的单突触DG电位，其次是双突触反应加上水槽位于内分子层，近端的EC诱导的水槽，其中DG内的关联突触的解剖研究证明。目前详细的地形研究的EC连接与DG在豚鼠证明与电生理方法的投影模式类似，即使不相同，在大鼠中的示踪技术所描述的。这份报告是必不可少的，为今后的研究动态paradecampal海马相互作用的豚鼠，特别是在分离的豚鼠脑制备。
Anatomical studies demonstrated that neurons located in the superficial layers of the medial and lateral aspects of the rat entorhinal cortex (EC) project to temporal and septal portions of both the dentate gyrus (DG) and the CA1 region of the hippocampus, respectively. In the present study we investigated with electrophysiological techniques the propagation pattern of different EC subfields to the DG of the in vitro isolated brain of the guinea-pig. Laminar field potential profiles from different portions of the DG were recorded with multi-channel silicon probes following direct stimulation of the ipsilateral EC surface performed in different positions under direct visual control. Current source density analysis of laminar profiles demonstrated that i) stimulation of the rostral-medial EC induced monosynaptic responses exclusively in the temporal pole of the DG, ii) stimulation of both the lateral and the caudal portions of the EC determined a diffuse monosynaptic activation of both the intermediate and septal DG. The regions of the EC that project to different sectors of the DG in the guinea-pig do not correlate to the EC subfields identified on the basis of cytoarchitectonic criteria. The EC-evoked monosynaptic DG potentials were followed by disynaptic responses coupled with sinks located in the inner molecular layer, proximal to the EC-induced sink, where intra-DG associative synapses were demonstrated by anatomical studies. The present detailed topographical study of the EC connections with the DG in the guinea-pig demonstrates with an electrophysiological approach a projection pattern similar, even if not identical, to that described with tracer techniques in the rat. This report is essential for future studies of the dynamic parahippocampal–hippocampal interactions in the guinea-pig, and in particular in the isolated guinea-pig brain preparation.