The support of matrix accumulation and the promotion of sheep articular cartilage defects repair in vivo by chitosan hydrogels
The support of matrix accumulation and the promotion of sheep articular cartilage defects repair in vivo by chitosan hydrogels
复制标题
壳聚糖水凝胶对基质积累的支持和促进绵羊关节软骨缺损的体内修复
DOI：
10.1016/j.joca.2009.08.007
复制
发表时间：
2010-02-01
影响因子：
7
通讯作者：
Wang, C. -Y.
中科院分区：
文献类型：
--
作者：
Hao, T.;Wen, N.;Wang, C. -Y.
Objective Chitosan has been widely used as an injectable scaffold in cartilage tissue engineering due to its characteristic biocompatibility and biodegradability In this study, chitosan was used in its hydrogel form as a scaffold for chondrocytes that act to reconstruct tissue-engineered cartilage and repair articular cartilage defects in the sheep model This study aims to find a novel way to apply chitosan in cartilage tissue engineeringMethods Temperature-responsive chitosan hydrogels were prepared by combining chitosan, beta-sodium glycerophosphate (GP) and hydroxyethyl cellulose (HEC) Tissue-engineered cartilage reconstructions were made in vitro by mixing sheep chondrocytes with a chitosan hydrogel. Cell survival and matrix accumulation were analyzed after 3 weeks in culture To collect data for in vivo repair, reconstructions cultured for 1 day were transplanted to the freshly prepared defects of the articular cartilage of sheep Then at both 12 and 24 weeks after transplantation, the grafts were extracted and analyzed histologically and immunohistochemicallyResults The results showed that the chondrocytes in the reconstructed cartilage survived and retained their ability to secrete matrix when cultured in vitro. Transplanted in vivo, the reconstructions repaired cartilage defects completely within 24 weeks. The implantation of chitosan hydrogels without chondrocytes also helps to repair cartilage defects.Conclusions The chitosan-based hydrogel could support matrix accumulation of chondrocytes and could repair sheep cartilage defects in 24 weeks. This study showcased the success of a new technique in its ability to repair articular cartilage defects. (C) 2009 Osteoarthritis Research Society International Published by Elsevier Ltd All rights reserved
