Regulated expression of Wnts and frizzleds at specific stages of follicular development in the rodent ovary
Regulated expression of Wnts and frizzleds at specific stages of follicular development in the rodent ovary
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DOI：
10.1210/en.143.3.898
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发表时间：
2002-03-01
期刊：
影响因子：
4.8
通讯作者：
Richards, JS
中科院分区：
文献类型：
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作者：
Hsieh, M;Johnson, MA;Richards, JS
Writ配体和Frizzled（Fz）G蛋白偶联受体影响细胞命运，包括卵巢的胚胎发育。由于这些调控分子在成年卵泡发育过程中的作用尚不清楚，因此进行了RT-PCR调查。Wnt-4、Fz-4和Fz-1是检测到的转录物，并且各自表现出特定的表达模式。Fz-1 mRNA在PMSG处理的小鼠排卵前卵泡中较低，但在人CG排卵峰后4-12 h内增加。通过原位分析，Fz-1转录本首先在卵泡膜细胞中增加，然后在排卵卵泡的颗粒细胞中增加，但在黄体中较低。与此相反，Wnt-4，在早期卵巢发育的关键因素，表达在小腔前卵泡。此外，在排卵前卵泡中检测到Wnt-4，并在黄体中表现出高水平。黄体中Wnt-4的潜在受体是Fz-4，其在该组织中也升高。虽然Wnt-4已被证明在其他组织中的PR下游发挥作用，但Wnt-4在不能排卵的PR缺失小鼠的卵泡中没有改变。相反，与正常同窝出生的小鼠相比，PR基因敲除小鼠卵巢中Fz-1的表达较低。因此，特定的Wnt/Fz在卵泡发育的不同阶段表达，表明该信号通路在卵巢中具有多种功能。
Writ ligands and Frizzled (Fz) G protein-coupled receptors impact cell fate, including embryonic development of the ovary. Because the role of these regulatory molecules during follicular development in the adult is not known, an RT-PCR survey was done. Wnt-4, Fz-4, and Fz-1 were among the transcripts detected, and each exhibited a specific pattern of expression. Fz-1 mRNA was low in preovulatory follicles of PMSG-treated mice but was increased within 4-12 h after an ovulatory surge of human CG. By in situ analysis, Fz-1 transcripts increased first in the theca cells and then in the granulosa cells of ovulating follicles but were low in corpora lutea. In contrast, Wnt-4, a critical factor in early ovarian development, was expressed in small preantral follicles. In addition, Wnt-4 was detected in preovulatory follicles and exhibited high levels in corpora lutea. A potential receptor for Wnt-4 in corpora lutea is Fz-4 that was also elevated in this tissue. Although Wnt-4 has been shown to function downstream of the PR in other tissues, Wnt-4 was not altered in follicles of PR-null mice that fail to ovulate. Rather expression of Fz-1 was lower in ovaries of PR knockout mice, compared with normal littermates. Thus, specific Wnt/Fz are expressed at distinct stages of follicular development, suggesting multiple functions for this signaling pathway in the ovary.