Protein determinants of insertional specificity for the Drosophila gypsy retrovirus.
Protein determinants of insertional specificity for the Drosophila gypsy retrovirus.
复制标题
果蝇吉普赛逆转录病毒插入特异性的蛋白质决定因素。
DOI：
10.1093/genetics/158.3.1101
复制
发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Corces,VG
中科院分区：
文献类型：
--
作者：
Labrador,M;Corces,VG
Thegypsyretrovirus invades the germ line of Drosophila females, inserting with a high frequency into theovolocus.Gypsyinsertion sites inovoare clustered within a region in the promoter of theovogene that contains multiple binding sites for the OvoA and OvoB proteins. We found that a 1.3-kb DNA fragment containing this region is able to confergypsyinsertional specificity independent of its genomic location. The frequency ofgypsyinsertions into theovogene is significantly lower in wild-type females than inovoD1females. In addition,gypsyinsertions inovoD1females occur during most stages of germ-line development whereas insertions in wild-type females occur only in late stages. This pattern of temporally specific insertions, as well as the higher frequency of insertion inovoD1females, correlates with the presence of the OvoA or OvoD1 proteins. The results suggest thatgypsyinsertional specificity might be determined by the binding of the OvoA repressor isoform to the promoter region of the gene.
