Wheat VIN3-like PHD finger genes are up-regulated by vernalization
Wheat VIN3-like PHD finger genes are up-regulated by vernalization
复制标题
DOI：
10.1007/s00438-006-0189-6
复制
发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Dubcovsky, Jorge
中科院分区：
文献类型：
--
作者：
Fu, Daolin;Dunbar, Mignon;Dubcovsky, Jorge
“春化”一词描述了植物暴露在较长时间的低温下后，营养阶段和生殖阶段之间的转换加速。在拟南芥中，春化通过沉默开花抑制基因开花位点C(FLC)来促进开花。FLC的有丝分裂稳定抑制是春化不敏感3(VIN3)和VIN3样蛋白(VIL)介导的染色质修饰的结果。在本研究中，我们从二倍体小麦中分离并鉴定了三个VIL基因，分别命名为TmVIL1、TmVIL2和TmVIL3。与拟南芥VIN3类似，三种小麦VIL蛋白都含有三个保守结构域，包括植物同源结构域Finger基序(PHD)、纤维连接蛋白III型结构域(FNIII)和VIN3相互作用结构域(VID)。遗传作图将TmVIL1、TmVIL2和TmVIL3基因座分别定位在5、6和1号染色体着丝粒区域。TmVIL1的染色体位置与春化基因VRN-D5相近，但需要更精确的定位信息来验证这种关系。春化可上调小麦VIL基因的转录，在低温处理4-6周后达到高峰。当转移回温暖条件时，小麦VIL基因的转录水平恢复到春化前的水平。此外，小麦VIL基因的转录水平还受到光周期的影响。这项研究表明，小麦VIL基因保留了与其拟南芥VIN3/VIL同源基因相似的结构和转录调控，这表明它们可能保留了一些功能。
The term 'vernalization' describes the acceleration of the transition between the vegetative and reproductive stages after exposing plants to an extended period of low temperature. In Arabidopsis, vernalization promotes flowering by silencing the flowering repressor gene FLOWERING LOCUS C (FLC). Mitotically stable repression of FLC is the result of chromatin modifications mediated by the Vernalization-INsensitive 3 (VIN3) and VIN3-Like (VIL) proteins. In this study, we identified and characterized three VIL genes in diploid wheat (Triticum monococcum L.), named TmVIL1, TmVIL2, and TmVIL3. Similar to Arabidopsis VIN3, all three wheat VIL proteins carry three conserved domains including a plant homeodomain finger motif (PHD), a fibronectin type III domain (FNIII), and a VIN3 interacting domain (VID). Genetic mapping placed TmVIL1, TmVIL2, and TmVIL3 loci in the centromeric regions of chromosome 5, 6, and 1, respectively. The chromosome location of TmVIL1 is close to that of the vernalization gene VRN-D5, but more precise mapping information is required to validate this relationship. Transcription of the wheat VIL genes was up-regulated by vernalization, with a peak after 4-6 weeks of cold treatment. When transferred back to warm conditions, transcript levels of the wheat VIL genes returned to pre-vernalization levels. In addition, the transcript levels of wheat VIL genes are affected by photoperiod. This study indicates that wheat VIL genes have retained a similar structure and transcriptional regulation as their Arabidopsis VIN3/VIL homologues, suggesting that they might have retained some of their functions.