On-Chip Fabrication of Paclitaxel-Loaded Chitosan Nanoparticles for Cancer Therapeutics
On-Chip Fabrication of Paclitaxel-Loaded Chitosan Nanoparticles for Cancer Therapeutics
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DOI：
10.1002/adfm.201301628
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发表时间：
2014-01-01
影响因子：
19
通讯作者：
Renaud, Philippe
中科院分区：
文献类型：
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作者：
Majedi, Fatemeh Sadat;Hasani-Sadrabadi, Mohammad Mahdi;Renaud, Philippe
证明了使用无溶剂微流体来微调用于药物输送的壳聚糖纳米颗粒的物理和化学性质。纳米粒子自组装是由水环境中的 pH 变化驱动的，通过避免传统技术常见的有机溶剂污染来提高生物相容性。在纳米颗粒自组装过程中控制混合时间（2.5-75 ms）使我们能够调整纳米颗粒的尺寸和表面电位，以最大限度地提高细胞吸收，从而显着提高药物有效性。这些纳米颗粒的紧凑纳米结构比以前的纳米颗粒更好地保留了药物效力，并且在生理pH下的长期循环过程中更加稳定。然而，当纳米粒子遇到肿瘤细胞并导致 pH 值下降时，药物成分就会被释放。此外，疏水性药物在纳米颗粒中的负载效率较之前的工作显着提高至95%以上。这里使用的微流体技术不仅可用于载药纳米颗粒的制造，还可用于更好地控制几乎任何自组装过程。
The use of solvent‐free microfluidics to fine‐tune the physical and chemical properties of chitosan nanoparticles for drug delivery is demonstrated. Nanoparticle self‐assembly is driven by pH changes in a water environment, which increases biocompatibility by avoiding organic solvent contamination common with traditional techniques. Controlling the time of mixing (2.5–75 ms) during nanoparticle self‐assembly enables us to adjust nanoparticle size and surface potential in order to maximize cellular uptake, which in turn dramatically increases drug effectiveness. The compact nanostructure of these nanoparticles preserves drug potency better than previous nanoparticles, and is more stable during long‐term circulation at physiological pH. However, when the nanoparticles encounter a tumor cell and the associated drop in pH, the drug contents are released. Moreover, the loading efficiency of hydrophobic drugs into the nanoparticles increases significantly from previous work to over 95%. The microfluidic techniques used here have applications not just for drug‐carrying nanoparticle fabrication, but also for the better control of virtually any self‐assembly process.