Colon carcinoma K-ras 2 oncogene of a familial polyposis coli patient.
Colon carcinoma K-ras 2 oncogene of a familial polyposis coli patient.
复制标题
家族性结肠息肉病患者的结肠癌 K-ras 2 癌基因。
DOI：
--
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Namba
中科院分区：
文献类型：
--
作者：
Y. Yuasa;M. Oto;C. Sato;M. Miyaki;T. Iwama;A. Tonomura;M. Namba
将结肠癌衍生细胞系（KMS-4）和家族性结肠息肉病患者皮肤成纤维细胞的DNA转染到NIH 3 T3细胞中，以检测与该疾病相关的癌基因。用正常皮肤成纤维细胞DNA没有观察到转化，而KMS-4细胞DNA能够转化NIH 3 T3细胞。通过与已知癌基因探针杂交，发现KMS-4转化基因是一种人激活的c-K-ras 2癌基因。对克隆的KMS-4 c-K-ras 2癌基因的序列分析表明，在第12位密码子处有一个由G到T的单核苷酸转换。这导致在该位置处半胱氨酸取代甘氨酸。用人工合成的标记寡核苷酸检测大肠癌细胞中第12位密码子的突变，发现了G → T的突变。这表明c-K-ras 2癌基因的激活可能与结肠癌的诱导有关。
The DNA of a colon carcinoma-derived cell line (KMS-4) and that of skin fibroblasts from a familial polyposis coli patient were transfected into NIH3T3 cells in order to detect oncogenes associated with the disease. No transformation was observed with the normal skin fibroblast DNA, while the KMS-4 cell DNA was able to transform NIH3T3 cells. Through hybridization with known oncogene probes, the KMS-4 transforming gene was found to be a human activated c-K-ras 2 oncogene. Sequence analysis of the molecularly cloned KMS-4 c-K-ras 2 oncogene showed a single nucleotide transition from G to T at the 12th codon. This results in substitution of cysteine for glycine at this position. On using labeled synthetic oligonucleotides to detect the mutation in codon 12, we found the G to T transition in colon carcinoma cells. This suggests that activation of the c-K-ras 2 oncogene could be associated with colon carcinoma induction.
人类 c-K-ras 癌基因的激活。
影响因子：
14.9
作者：
Yamamoto,F;Perucho,M
通讯作者：
Perucho,M