Immune reconstitution following bone marrow transplantation: comparison of recipients of T-cell depleted marrow with recipients of conventional marrow grafts
Immune reconstitution following bone marrow transplantation: comparison of recipients of T-cell depleted marrow with recipients of conventional marrow grafts
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骨髓移植后的免疫重建：T细胞耗尽骨髓受者与传统骨髓移植受者的比较
DOI：
10.1182/blood.v73.5.1340.bloodjournal7351340
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发表时间：
1989
期刊：
影响因子：
20.3
通讯作者：
R. O'reilly
中科院分区：
文献类型：
--
作者：
C. Keever;T. Small;N. Flomenberg;G. Heller;K. Pekle;P. Black;A. Pecora;A. Gillio;N. Kernan;R. O'reilly
对34例白血病患者进行了常规骨髓移植（BMT）和52例去T细胞骨髓移植（BMT）后造血细胞的重建和免疫功能的体外测定。通过植入当天或通过第100天的细胞绝对数量测量，未观察到T细胞耗竭对骨髓或淋巴细胞恢复的影响。正常数量的溶细胞活性的自然杀伤细胞恢复最早，并迅速其次在两组患者的循环B细胞的外观和正常化的反应，B细胞有丝分裂原。然而，正常T细胞增殖反应的恢复在T细胞耗竭移植物的受体中更延迟。仅在移植后的前3个月内观察到显著的定量差异。两移植组之间的CD 3 + T细胞数量和CD 4：CD 8阳性细胞比例均无显著差异。有丝分裂原诱导的外周血淋巴细胞（PBL）的T细胞耗竭骨髓移植后的免疫球蛋白的生产量低于传统的骨髓受体，通过第一年，低正常IgM生产达到4至6个月，在两组。IgG的产生在常规BMT后7至9个月达到正常水平，但在任何一种移植后1年才保持在这一水平。从中性粒细胞绝对计数达到500到移植后第180天，对感染发生率的评估显示两组之间没有显著差异;事实上，常规骨髓受体的总体非白血病死亡率较高。因此，在我们的系列研究中，从骨髓移植物中去除成熟细胞并不影响骨髓和淋巴细胞的恢复速度或程度，但确实影响体外T细胞依赖性功能的再生。我们注意到早期的数量差异和延迟正常化的T细胞功能的测量，而不是长期的绝对缺陷。体外缺陷未导致两组之间存在显著的临床明显差异。
The reconstitution of hematopoietic cells and in vitro assays of immunologic function have been followed in leukemic patients after conventional bone marrow transplantation (BMT) (N = 34) and T-cell depleted BMT (N = 52) from human leukocyte antigen (HLA)-identical sibling donors. No effects of the T-cell depletion could be seen on the recovery of myeloid or lymphoid cells as measured by the day to engraftment or by the absolute number of cells through day 100. Normal numbers of lytically active natural killer cells returned the earliest and were rapidly followed in both groups of patients by the appearance of circulating B cells and normalization of the responses to B-cell mitogens. However, the recovery of normal T-cell proliferative responses were more delayed in recipients of T-cell depleted grafts. Significant quantitative differences were seen only during the first 3 months after transplantation. Neither the number of CD3+ T cells nor the ratio of CD4:CD8 positive cells differed markedly between the two transplant groups. Mitogen-induced immunoglobulin production by peripheral blood lymphocytes (PBL) from patients following T-cell depleted BMT was quantitatively less than that of conventional marrow recipients through the first year, with low normal IgM production reached by 4 to 6 months in both groups. IgG production reached low normal 7 to 9 months after conventional BMT but did not remain at this level until 1 year following either type of transplant. Assessment of the incidence of infections from the day the absolute neutrophil count reached 500 until day 180 after transplant revealed no significant differences between the two groups; indeed, the overall nonleukemic mortality was higher in the recipients of conventional bone marrow. Thus, in our series, the removal of mature cells from the marrow graft did not affect the rate or degree of recovery of myeloid and lymphoid cells but did affect the regeneration of in vitro T-cell dependent functions. We noted early quantitative differences and a delay in the normalization of the T-cell functions measured rather than prolonged absolute deficiencies. The in vitro deficiencies did not result in significant clinically apparent differences between the two groups.
T 细胞耗尽的骨髓移植受者中功能性 T 细胞频率的恢复迟缓。
影响因子：
20.3
作者：
Daley,JP;Rozans,MK;Smith,BR;Burakoff,SJ;Rappeport,JM;Miller,RA
通讯作者：
Miller,RA