Influence of follicular fluid GDF9 and BMP15 on embryo quality
Influence of follicular fluid GDF9 and BMP15 on embryo quality
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DOI：
10.1016/j.fertnstert.2011.03.045
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发表时间：
2011-06-01
影响因子：
6.7
通讯作者：
Atabey, Nese
中科院分区：
文献类型：
--
作者：
Gode, Funda;Gulekli, Bulent;Atabey, Nese
目的：为了评估卵泡液中前肽水平和成熟形式的生长分化因子（GDF）9和骨形态发生蛋白（BMP）15与随后的卵母细胞和胚胎质量之间的关联。设计：前瞻性临床研究。设置：大学医院。患者：81名接受体外受精（IVF）/卵胞浆内单精子注射（ICSI）的不孕患者。干预措施：通过蛋白质印迹分析确定卵泡液GDF 9和BMP 15的前肽和成熟形式的表达水平。结果：成熟GDF 9水平与卵母细胞核成熟度呈显著正相关（P < 0. 05），成熟GDF 9水平与卵母细胞核成熟度呈显著正相关（P < 0. 05），成熟GDF 9水平与卵母细胞核成熟度呈显著正相关（P < 0. 05）。高胚胎质量组的平均成熟GDF 9水平为4.87 +/- 0.60，低胚胎质量组为1.45 +/- 0.81。在前肽GDF 9和BMP 15表达状态方面，患者之间的胚胎质量没有统计学显著差异。卵泡液中P水平与GDF 9成熟水平呈负相关。结论：卵泡液中GDF 9成熟水平与卵母细胞核成熟及胚胎质量密切相关。（Fertil Steril（R）2011;95：2274-8.（C）2011年由美国生殖医学协会。
Objective: To evaluate the association between follicular fluid levels of propeptide and mature forms of growth differentiation factor (GDF) 9 and bone morphogenetic protein (BMP) 15 with subsequent oocyte and embryo quality.Design: Prospective clinical study.Setting: University hospital.Patient(s): Eighty-one infertile patients who underwent in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI).Intervention(s): The expression levels of the propeptide and mature forms of follicular fluid GDF9 and BMP15 were determined by western blot analysis. The levels of follicular fluid hormones (FSH, E-2, and P) were measured with automated chemiluminescent enzyme immunoassays.Main Outcome Measure(s): The relationships between the levels of GDF9 and BMP15, hormones, oocyte maturation, and embryo quality.Result(s): Mature GDF9 levels were significantly correlated with the nuclear maturation of oocytes. The mean mature GDF9 level was 4.87 +/- 0.60 in the high-embryo-quality group and 1.45 +/- 0.81 in the low-embryo-quality group. There were no statistically significant differences in embryo quality among the patients regarding propeptide GDF9 and BMP15 expression status. There was a negative correlation between follicular fluid levels of P and the mature form of GDF9.Conclusion(s): Higher mature GDF9 levels in the follicular fluid were significantly correlated with oocyte nuclear maturation and embryo quality. (Fertil Steril (R) 2011;95:2274-8. (C)2011 by American Society for Reproductive Medicine.)