Fabrication of gelatin-hyaluronic acid hybrid scaffolds with tunable porous structures for soft tissue engineering
Fabrication of gelatin-hyaluronic acid hybrid scaffolds with tunable porous structures for soft tissue engineering
复制标题
用于软组织工程的具有可调多孔结构的明胶-透明质酸混合支架的制造
DOI：
10.1016/j.ijbiomac.2011.01.012
复制
发表时间：
2011-04-01
影响因子：
8.2
通讯作者：
Wang, Jinwu
中科院分区：
文献类型：
--
作者：
Zhang, Fan;He, Chuang Long;Wang, Jinwu
具有高度开放多孔结构的三维支架的开发是组织工程领域的重要课题之一。本研究通过冷冻干燥明胶/透明质酸（GE/HA）共混溶液，然后用1-乙基-3-（3-二甲氨基丙基）碳二亚胺盐酸盐（EDC）进行化学交联，制备了具有不同孔隙形态的三维大孔明胶/HA杂化支架。用扫描电镜（SEM）和傅里叶变换红外光谱（FTIR）对所得支架进行了表征。研究了它们的溶胀性能、体外降解性能和抗压强度。为了评价支架的体外细胞相容性，将小鼠L929成纤维细胞接种到支架上进行细胞形态学和细胞活力研究。结果表明，明胶与HA的比例可以调控支架的多孔结构，杂化支架的溶胀率和降解速率均随HA含量的增加而增加，EDC交联支架提高了支架在培养基中的抗降解能力，提高了支架的机械强度。体外实验结果表明，所制备的支架不会诱导细胞毒性效应，适合细胞生长，尤其是在明胶含量较高的支架的情况下。理化和生物学研究的综合结果表明，开发的GE/HA杂化支架具有良好的潜力和生物相容性的软组织工程应用。(C)2011爱思唯尔有限公司版权所有。
The development of three-dimensional (3-D) scaffolds with highly open porous structure is one of the most important issues in tissue engineering. In this study, 3-D macroporous gelatin/hyaluronic acid (GE/HA) hybrid scaffolds with varying porous morphology were prepared by freeze-drying their blending solutions and subsequent chemical crosslinking by using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The resulting scaffolds were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Their swelling, in vitro degradation properties and compressive strength were also investigated. To evaluate in vitro cytocompatibility of scaffolds, mouse L929 fibroblasts were seeded onto the scaffolds for cell morphology and cell viability studies. It was found that the porous structure of scaffolds can be tailored by varying the ratios of gelatin to HA, both the swelling ratios and degradation rate increased with the increase of HA content in hybrid scaffolds, and crosslinking the scaffolds with EDC improved the degradation resistance of the scaffold in culture media and increased the mechanical strength of scaffolds. The in vitro results revealed that the prepared scaffolds do not induce cytotoxic effects and suitable for cell growth, especially in the case of scaffolds with higher gelatin content. The combined results of the physicochemical and biological studies suggested that the developed GE/HA hybrid scaffolds exhibit good potential and biocompatibility for soft tissue engineering applications. (C) 2011 Elsevier B.V. All rights reserved.